Zhejiang Chinese Medicine University, Hangzhou, 310053, China.
Zhejiang Chinese Medicine University, Hangzhou, 310053, China.
Biomed Pharmacother. 2017 Oct;94:88-92. doi: 10.1016/j.biopha.2017.07.076. Epub 2017 Jul 26.
The aim of the present study was to investigate the protective effect of the total flavonoids (TFs) from the leaves of Carya cathayensis Sarg. against early development of atherosclerosis. An in vivo model of carotid arterial partial ligation was established in mice, and the effects of TFs were investigated by morphometric measurements, Cell proliferation measurement and immunohistochemistry. The results showed that TFs could reduce neointima area by 41%, and the adventitial thickening induced by partial ligation was remarkable inhibited by TFs treatment. medial SMCs proliferation was significantly inhibited in TFs treated group. Immunohistochemistry analyses demonstrated that mice with TFs treatment have significant less macrophages accumulation in adventitia. These findings indicated that TFs have inhibitory effect in early atherosclerosis lesion formation model and strong action on reduce the inflammation in vivo.
本研究旨在探讨山核桃叶总黄酮（TFs）对动脉粥样硬化早期发展的保护作用。建立了小鼠颈总动脉部分结扎的体内模型，通过形态计量学测量、细胞增殖测量和免疫组织化学方法研究了 TFs 的作用。结果表明，TFs 可使新生内膜面积减少 41%，并显著抑制 TFs 处理引起的外膜增厚。TFs 处理组中，中层平滑肌细胞增殖明显受到抑制。免疫组织化学分析表明，TFs 治疗组小鼠外膜中巨噬细胞的积累明显减少。这些发现表明，TFs 对早期动脉粥样硬化病变形成模型具有抑制作用，并具有体内减轻炎症的强大作用。
