Pierce Janet D, Shen Qiuhua, Peltzer Jill, Thimmesch Amanda, Hiebert John B
School of Nursing, University of Kansas, Kansas City, KS.
School of Nursing, University of Kansas, Kansas City, KS.
Nurs Outlook. 2017 Sep-Oct;65(5S):S44-S52. doi: 10.1016/j.outlook.2017.06.012. Epub 2017 Jul 1.
Traumatic brain injury is a major cause of morbidity and mortality that affects military service members and veterans.
Explore the effects of ubiquinol before traumatic brain injury on cerebral gene expression to elucidate molecular mechanisms of ubiquinol neuroprotection.
In this experimental study, Fisher rats in the untreated (n = 2) and ubiquinol-treated (n = 2) groups received respectively either normal saline or ubiquinol 30 min before traumatic brain injury induced by controlled cortical impact. Ribonucleic acid sequencing and ingenuity pathway analysis were conducted to detect cerebral gene and signaling expression profiles.
In the ubiquinol-treated group, 67 ingenuity pathway analysis transcripts in the ubiquinol-treated group were statistically different from those in the untreated group (p <.0001).
Administering ubiquinol 30 min before traumatic brain injury significantly affected cerebral gene expression profiles that may be involved in the most fundamental molecular mechanisms of bioenergetics and free radical production.
创伤性脑损伤是影响军人和退伍军人发病和死亡的主要原因。
探讨创伤性脑损伤前泛醇对脑基因表达的影响，以阐明泛醇神经保护的分子机制。
在本实验研究中，未治疗组（n = 2）和泛醇治疗组（n = 2）的Fisher大鼠在通过控制性皮质撞击诱导创伤性脑损伤前30分钟分别接受生理盐水或泛醇。进行核糖核酸测序和 Ingenuity 通路分析以检测脑基因和信号表达谱。
在泛醇治疗组中，泛醇治疗组的67个 Ingenuity 通路分析转录本与未治疗组有统计学差异（p <.0001）。
创伤性脑损伤前30分钟给予泛醇显著影响脑基因表达谱，这些表达谱可能涉及生物能量学和自由基产生的最基本分子机制。
