Osman Essam Eldin A
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:469-477. doi: 10.1016/j.saa.2017.07.024. Epub 2017 Jul 19.
This work represents a comparative study of different approaches of manipulating ratio spectra, applied on a binary mixture of ciprofloxacin HCl and dexamethasone sodium phosphate co-formulated as ear drops. The proposed new spectrophotometric methods are: ratio difference spectrophotometric method (RDSM), amplitude center method (ACM), first derivative of the ratio spectra (DD) and mean centering of ratio spectra (MCR). The proposed methods were checked using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical formulation containing the cited drugs. The proposed methods were validated according to the ICH guidelines. A comparative study was conducted between those methods regarding simplicity, limitations and sensitivity. The obtained results were statistically compared with those obtained from the reported HPLC method, showing no significant difference with respect to accuracy and precision.
本研究对用于盐酸环丙沙星和地塞米松磷酸钠二元混合耳用制剂的不同比率光谱处理方法进行了比较研究。所提出的新分光光度法有：比率差分光光度法（RDSM）、振幅中心法（ACM）、比率光谱的一阶导数（DD）和比率光谱的均值中心化（MCR）。所提出的方法通过实验室制备的混合物进行了检验，并成功应用于含有上述药物的药物制剂分析。所提出的方法根据国际人用药品注册技术协调会（ICH）指南进行了验证。对这些方法在简便性、局限性和灵敏度方面进行了比较研究。将所得结果与报道的高效液相色谱法（HPLC）所得结果进行了统计学比较，结果表明在准确性和精密度方面无显著差异。
