一种用于多能性的多重筛选方法。

A multiplexed screening method for pluripotency.

作者信息

Plotnikov Alexander, Kozer Noga, Krupalnik Vladislav, Peles Shani, Mor Nofar, Rais Yoach, Hanna Jacob H, Barr Haim M

机构信息

The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel.

The Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Stem Cell Res. 2017 Aug;23:158-162. doi: 10.1016/j.scr.2017.07.014. Epub 2017 Jul 12.

DOI:10.1016/j.scr.2017.07.014

PMID:28756340

Abstract

Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available reagents enabling quantification of ALP levels and cell number by two sequential luminescence readouts. Using this method we were able to detect and analyze somatic reprogramming into pluripotent stem cells. The method is highly applicable for High Throughput Screening (HTS) campaigns and analysis.

摘要

碱性磷酸酶（ALP）水平的测定是临床和基础研究中广泛使用的方法。我们提出了一种简单且成本低廉的基于发光的方法，该方法能够在一个样品孔中对细胞内ALP水平进行多重测量和标准化。该方法包括两种市售试剂，通过两次连续的发光读数实现对ALP水平和细胞数量的定量。使用这种方法，我们能够检测和分析体细胞重编程为多能干细胞的过程。该方法非常适用于高通量筛选（HTS）活动和分析。