Yotov S, Atanasov A
Department of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
Merichleri Buffalo Farm, Merichleri, Stara Zagora, Bulgaria.
Reprod Domest Anim. 2018 Feb;53(1):20-25. doi: 10.1111/rda.13046. Epub 2017 Jul 30.
This study was designed to evaluate the dominant follicles development and the estradiol-17β concentrations in non-ovulating and ovulating post-partum buffaloes. Sixteen Bulgarian Murrah buffaloes were submitted to transrectal ultrasonographic examination from the 1st post-partum day until day 50, 3 days apart. The follicular diameter of the different categories of follicles and the ovulations was recorded. The animals were allocated into two groups: I (n = 6) non-ovulating and II (n = 10) ovulating buffaloes. Serum estradiol-17β concentrations on the days for dominant follicle registration were measured by enzyme-linked immunosorbent assay. The results were statistically processed by analysis of variance, non-parametric and correlation analysis. The mean intervals between calving and first dominant follicle detection differed significantly (p < .05) among the groups (19.5 ± 6.2 vs. 13.8 ± 5.1 days), while the mean intervals between registered dominant follicles from two successive waves were comparable. The mean follicular diameters for the same category follicles in both groups were similar. Different estradiol-17β concentrations (p < .05) for the first dominant follicle between non-ovulating (23.5 ± 7.0 pg/ml) and ovulating (33.3 ± 8.4 pg/ml) buffaloes were determined. The cumulative percentages of buffaloes with firstly detected dominant follicle and ovulating animals correlated positively (r ≥ .84; p < .05) to post-partum days. In conclusion, non-ovulating and ovulating post-partum Bulgarian Murrah buffaloes showed differences in the development of the first dominant follicle and estradiol-17β concentrations during the time of dominant follicles detection.
本研究旨在评估产后不排卵和排卵的水牛优势卵泡发育情况及雌二醇-17β浓度。16头保加利亚穆拉水牛从产后第1天至第50天，每隔3天接受经直肠超声检查。记录不同类别卵泡的直径和排卵情况。将动物分为两组：I组（n = 6）不排卵水牛和II组（n = 10）排卵水牛。通过酶联免疫吸附测定法测量优势卵泡记录日的血清雌二醇-17β浓度。结果采用方差分析、非参数分析和相关分析进行统计学处理。两组间产犊至首次检测到优势卵泡的平均间隔差异显著（p <.05）（19.5 ± 6.2天 vs. 13.8 ± 5.1天），而连续两个波次记录的优势卵泡之间的平均间隔相当。两组中相同类别卵泡的平均直径相似。测定了不排卵（23.5 ± 7.0 pg/ml）和排卵（33.3 ± 8.4 pg/ml）水牛首次优势卵泡的不同雌二醇-17β浓度（p <.05）。首次检测到优势卵泡的水牛和排卵动物的累积百分比与产后天数呈正相关（r≥.84；p <.05）。总之，产后不排卵和排卵的保加利亚穆拉水牛在首次优势卵泡发育及优势卵泡检测期间的雌二醇-17β浓度方面存在差异。
