暴露于饥饿和水族箱应激下的鲽（欧洲比目鱼）的血清皮质醇、葡萄糖和脂质

Serum cortisol, glucose and lipids in plaice (Pleuronectes platessa L.) exposed to starvation and aquarium stress.

作者信息

White A, Fletcher T C

出版信息

Comp Biochem Physiol A Comp Physiol. 1986;84(4):649-53. doi: 10.1016/0300-9629(86)90380-4.

Abstract

Plaice were maintained in the aquarium (11-12 degrees C) during May for 15 days without feeding. Within 48 hr, there was a decline in serum total lipids (P less than 0.001), phospholipids (P less than 0.01), triglycerides (P less than 0.001), cortisol (P less than 0.01) and glucose (P less than 0.001), but an increase in nonesterified fatty acids (NEFA; P less than 0.01). There was a significant inverse correlation between NEFA and glucose over 15 days (P less than 0.001) and between NEFA and cortisol over the first 5 days (P less than 0.01). Cortisol and glucose showed a significant correlation over 15 days (P less than 0.01). Serum cortisol and glucose were not apparently affected by starvation. Only cortisol provided a sensitive indicator of aquarium disturbance. Exposure of the fish to agitation or reduced O2 for 1 hr significantly elevated cortisol (P less than 0.001) but only the latter treatment elevated glucose (P less than 0.01); neither treatment affected the lipids.

摘要

5月期间，将鲽鱼饲养在水族箱中（11 - 12摄氏度），禁食15天。在48小时内，血清总脂质（P < 0.001）、磷脂（P < 0.01）、甘油三酯（P < 0.001）、皮质醇（P < 0.01）和葡萄糖（P < 0.001）含量下降，但非酯化脂肪酸（NEFA；P < 0.01）含量增加。在15天内，NEFA与葡萄糖之间存在显著负相关（P < 0.001），在前5天内，NEFA与皮质醇之间存在显著负相关（P < 0.01）。在15天内，皮质醇与葡萄糖呈现显著相关性（P < 0.01）。血清皮质醇和葡萄糖未明显受到饥饿影响。只有皮质醇是水族箱干扰的敏感指标。将鱼暴露于搅动环境或低氧环境1小时，可显著提高皮质醇含量（P < 0.001），但只有后一种处理方式可提高葡萄糖含量（P < 0.01）；两种处理方式均未影响脂质含量。