McLean E, Ash R
Comp Biochem Physiol A Comp Physiol. 1986;84(4):687-90. doi: 10.1016/0300-9629(86)90388-9.
A sensitive enzyme-linked immunosorbent assay (ELISA) technique was used in order to determine horseradish peroxidase (HRP) uptake from the carp gut. HRP was detected in blood plasma and various tissues within 15 min of oral intubation. The time-course of net accumulation (uptake-degradation) over a 2 hr period was recorded. The presence of HRP reached a maximum in the body tissues approximately 1 hr after intubation and on a microgram/g wet weight basis the order of accumulation within the tissues was spleen greater than kidney greater than liver. The total organ accumulation (net) was in the order liver greater than kidney greater than spleen.
为了测定鲤鱼肠道对辣根过氧化物酶（HRP）的摄取，采用了一种灵敏的酶联免疫吸附测定（ELISA）技术。经口插管后15分钟内，在血浆和各种组织中检测到了HRP。记录了2小时内净积累（摄取-降解）的时间进程。插管后约1小时，HRP在身体组织中的含量达到最高，以微克/克湿重计，组织内积累顺序为脾脏＞肾脏＞肝脏。器官总积累量（净积累）顺序为肝脏＞肾脏＞脾脏。
