Josiassen Michael Vallop, Charabi Birgitte, Lajer Christel Braemer, Garnaes Emilie, Hamilton Therkildsen Marianne, Norrild Bodil, Specht Lena, Nielsen Finn Cilius, von Buchwald Christian
a Department of Otorhinolaryngology, Head and Neck Surgery and Audiology , Rigshospitalet, University of Copenhagen , Copenhagen , Denmark.
b Department of Pathology , Rigshospitalet, University of Copenhagen , Copenhagen , Denmark.
Acta Otolaryngol. 2017 Dec;137(12):1260-1264. doi: 10.1080/00016489.2017.1356934. Epub 2017 Jul 30.
Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor.
Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC.
Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%).
In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.
人乳头瘤病毒（HPV）已知与口咽鳞状细胞癌（OPSCC）相关，并且可能在肿瘤转移中发挥至关重要的作用。本研究的目的是将宫颈淋巴结转移灶的HPV状态与其各自的原发性OPSCC肿瘤进行关联。
对34例宫颈淋巴结转移患者的福尔马林固定、石蜡包埋（FFPE）组织样本进行HPV 16 DNA聚合酶链反应（PCR）、p16免疫组织化学和HPV分型分析。将结果与OPSCC原发性肿瘤中发现的HPV状态和类型进行关联。
比较HPV DNA状态和p16，我们发现21例原发性肿瘤和淋巴结转移灶为HPV阳性（61.8%），7例原发性肿瘤和淋巴结转移灶为HPV阴性（20.6%）。在关联原发性肿瘤和淋巴结转移时，6例患者样本存在差异（17.6%）。
在本研究中，绝大多数情况下OPSCC及其宫颈淋巴结转移灶中的HPV状态相关。然而，HPV检测方法可能存在一定局限性，导致不同程度的不相关性。在根据HPV状态进行治疗分层时应考虑到这一点。
