袖状胃切除术对肥胖大鼠代谢谱及脂肪组织中肾素-血管紧张素系统表达的影响。

Effects of Sleeve Gastrectomy on the Metabolic Profile and on the Expression of Renin-Angiotensin System in Adipose Tissue of Obese Rats.

作者信息

Crespo Thaisa Soares, Andrade Joao Marcus Oliveira, Paraiso Alanna Fernandes, de Farias Lelis Deborah, Chagas Pablo Vinicyus Ferreira, Jorge Antonio Sergio Barcala, Ferreira Wagner Leite, de Paula Alfredo Mauricio Batista, Guimaraes Andre Luiz Sena, Santos Sergio Henrique Sousa

机构信息

Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.

Department of Surgery, Hospital Santa Casa - Irmandade Nossa Senhora Merces, Montes Claros, Minas Gerais, Brazil.

出版信息

Protein Pept Lett. 2017 Nov 17;24(9):861-868. doi: 10.2174/0929866524666170728155926.

DOI:10.2174/0929866524666170728155926

PMID:28758596

Abstract

BACKGROUND

Sleeve gastrectomy (SG) has been used as a multipurpose surgical procedure for the treatment of obesity.

OBJECTIVES

The present study aimed to assess the effects of SG on the metabolic and inflammatory profile and renin-angiotensin system (RAS) expression in the white adipose tissue of male rats with obesity induced by a high-fat diet.

METHODS

Male Wistar rats were treated with a standard diet or high-fat diet and submitted to SG or sham surgery. The glycemic and lipid profiles and gene expression of inflammatory markers and RAS components in adipose tissue were evaluated.

RESULTS

SG led to weight loss, decreased adiposity (p < 0.01) and a reduction in plasma glucose (p < 0.05), C-peptide (p < 0.05), insulin (p < 0.001) and total cholesterol (p < 0.05) levels. In addition, SG led to a decrease in the expression of tumor necrosis factor-alpha (TNF-α) (p < 0.01), interleukin- 6 (IL-6) (p < 0.001), angiotensinogen (AGT) (p < 0.001) and angiotensin converting enzyme (ACE) (p < 0.05) and increased the expression of angiotensin converting enzyme 2 (ACE2) (p < 0.05) in white adipose tissue. No statistically significant differences were observed for AT1 (p = 0.10) and Mas (p = 0.22) receptors.

CONCLUSION

This study showed that SG leads to weight loss and improves metabolic parameters. Changes in the expression of RAS components and of inflammatory molecules in adipose tissue seem to play a role the before beneficial effects of the SG.

摘要

背景

袖状胃切除术（SG）已被用作治疗肥胖症的多用途外科手术。

目的

本研究旨在评估SG对高脂饮食诱导的肥胖雄性大鼠白色脂肪组织中代谢和炎症谱以及肾素-血管紧张素系统（RAS）表达的影响。

方法

雄性Wistar大鼠接受标准饮食或高脂饮食，并接受SG或假手术。评估血糖和血脂谱以及脂肪组织中炎症标志物和RAS成分的基因表达。

结果

SG导致体重减轻、肥胖程度降低（p < 0.01），血浆葡萄糖（p < 0.05）、C肽（p < 0.05）、胰岛素（p < 0.001）和总胆固醇（p < 0.05）水平降低。此外，SG导致白色脂肪组织中肿瘤坏死因子-α（TNF-α）（p < 0.01）、白细胞介素-6（IL-6）（p < 0.001）、血管紧张素原（AGT）（p < 0.001）和血管紧张素转换酶（ACE）（p < 0.05）的表达降低，血管紧张素转换酶2（ACE2）的表达增加（p < 0.05）。AT1（p = 0.10）和Mas（p = 0.22）受体未观察到统计学上的显著差异。