Bower D J, Jeppesen P
Exp Cell Res. 1986 Nov;167(1):166-76. doi: 10.1016/0014-4827(86)90214-4.
Serum from an individual with the CREST syndrome (calcinosis, Raynaud's phenomenon, esophageal dismotility, sclerodactyly, telangiectasia) reacts not only with kinetochores, but also with a cytoplasmic, phosphorylatable polypeptide, which is shown by immunofluorescence in whole cells and immunoelectronmicroscopy in sections to be associated with actin stress fibres in cultured mammalian cells. The antigen shows some variation in molecular weight between species, estimated by immunoblotting to range from 68 to 76 kD between mouse, Chinese hamster, sheep and human cells. Much of the polypeptide copurifies with coated vesicles, of which approx. 5% bound antibody from the serum, as detected by immunogold electronmicroscopy.
来自患有CREST综合征（钙质沉着、雷诺现象、食管动力障碍、指端硬化、毛细血管扩张）个体的血清不仅与动粒发生反应，还与一种细胞质的、可磷酸化的多肽发生反应，通过全细胞免疫荧光和切片免疫电子显微镜显示，该多肽在培养的哺乳动物细胞中与肌动蛋白应力纤维相关。通过免疫印迹估计，该抗原在不同物种间分子量有一定差异，在小鼠、中国仓鼠、绵羊和人类细胞之间范围为68至76 kD。该多肽大部分与被膜小泡共纯化，通过免疫金电子显微镜检测，其中约5%结合了血清中的抗体。
