Animal Health Department, Faculty of Veterinary Medicine, Campus Universitario de Rabanales, 'International Excellence Agrifood Campus, ceiA3', Córdoba University, 14071 Córdoba, Spain; CICAP - Food Research Centre, 14400 Pozoblanco, Córdoba, Spain.
Animal Health Department, Faculty of Veterinary Medicine, Campus Universitario de Rabanales, 'International Excellence Agrifood Campus, ceiA3', Córdoba University, 14071 Córdoba, Spain.
Int J Food Microbiol. 2017 Oct 3;258:68-72. doi: 10.1016/j.ijfoodmicro.2017.07.016. Epub 2017 Jul 26.
The safety of ready-to-eat products such as cured pork loins must be guaranteed by the food industry. In the present study, the efficacy of the dry curing process of pork loins obtained from free-range pigs in the reduction of three of the most important foodborne pathogens is analysed. A total of 28 pork loin segments, with an average weight of 0.57±0.12kg, were divided into four groups with three being inoculated by immersion with 7logCFU/ml of either Salmonella Typhimurium, Campylobacter coli or Listeria innocua and the last one inoculated by immersion with sterile medium (control group). The loin segments were treated with a seasoning mixture of curing agents and spices, packed in a synthetic sausage casing and cured for 64days. Microbiological analysis, pH and water activity (a) were assessed at four stages. The values of pH and a decreased with curing time as expected. S. Typhimurium and C. coli dropped significantly (3.28 and 2.14 log units, respectively), but limited reduction of L. innocua (0.84 log unit) was observed along the curing process. In our study, three factors were considered critical: the initial concentration of the bacteria, the progressive reduction of pH and the reduction of a values. Our results encourage performing periodic analysis at different stages of the manufacturing of dry cured pork loins to ensure the absence of the three evaluated foodborne pathogens.
必须由食品行业保证即食产品(如腌制猪里脊肉)的安全性。在本研究中,分析了来自自由放养猪的腌制猪里脊肉干腌制过程对三种最重要食源性致病菌的减少效果。总共将 28 个猪里脊段(平均重量为 0.57±0.12kg)分为四组,三组通过浸泡用 7logCFU/ml 的肠炎沙门氏菌、大肠杆菌或无害李斯特菌接种,最后一组通过浸泡无菌培养基(对照组)接种。将里脊段用腌制剂和香料的调味混合物处理,包装在合成香肠肠衣中并腌制 64 天。在四个阶段评估微生物分析、pH 值和水活度(a)。pH 值和 a 值随着腌制时间的推移而降低,这是预期的结果。肠炎沙门氏菌和大肠杆菌的数量显著下降(分别下降了 3.28 和 2.14 个对数单位),但在腌制过程中,无害李斯特菌的减少有限(0.84 个对数单位)。在我们的研究中,有三个因素被认为是关键因素:细菌的初始浓度、pH 值的逐渐降低和 a 值的降低。我们的结果鼓励在干腌猪里脊肉制造的不同阶段进行定期分析,以确保不存在三种评估的食源性致病菌。