Li Jun, Zheng Dayong, Zhen Bixian, Sun Yunfeng, Chen Fen, Zhan Kaixuan, Wei Ning, Zhang Lide
Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
Planta Med. 2018 Jan;84(2):111-116. doi: 10.1055/s-0043-117414. Epub 2017 Jul 31.
Bruceantinol (BOL), a quassinoid compound isolated from the fruits of , has been reported to have cytotoxic and antibacterial effects. In this study, a rapid, sensitive, and specific ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed for the quantitative determination of BOL in rat plasma. The samples were treated by simple liquid-liquid extraction with ethyl acetate and separated on an UPLC BEH C18 column (2.1 mm × 50 mm) using a 3-min gradient elution scheme, which consists of water (0.1% v/v, formic acid) and methanol (0.1%, v/v, formic acid) to achieve the separation of BOL and sinomenine (IS) with high selectivity. The electrospray ionization source was used in positive ion mode; the multiple reaction monitoring quantified the target fragment ions 629.6 → 569.5 for BOL and 330.5 → 207.3 for IS. This work was evaluated with regards to the specificity, extraction recovery, matrix effect, linearity, accuracy, precision, stability, and dilution integrity. This approach was used to examine the pharmacokinetics of BOL in rats after oral (0.3 mg/kg) and intravenous (0.15 mg/kg) administration. BOL presented fast excretion and very low oral bioavailability.
布鲁斯抗诺醇（BOL）是一种从植物果实中分离得到的苦木素类化合物，据报道具有细胞毒性和抗菌作用。在本研究中，建立了一种快速、灵敏且特异的超高效液相色谱 - 串联质谱（UPLC-MS/MS）方法，用于定量测定大鼠血浆中的BOL。样品采用乙酸乙酯简单液 - 液萃取法处理，并在UPLC BEH C18柱（2.1 mm×50 mm）上进行分离，使用3分钟梯度洗脱方案，该方案由水（0.1% v/v，甲酸）和甲醇（0.1%，v/v，甲酸）组成，以实现BOL和青藤碱（内标）的高选择性分离。采用电喷雾电离源，正离子模式；多反应监测对目标碎片离子进行定量，BOL的为629.6 → 569.5，内标的为330.5 → 207.3。对该方法的特异性、萃取回收率、基质效应、线性、准确性、精密度、稳定性和稀释完整性进行了评估。该方法用于研究大鼠口服（0.3 mg/kg）和静脉注射（0.15 mg/kg）BOL后的药代动力学。BOL表现出快速排泄和极低的口服生物利用度。
原文中“the fruits of ”后面缺少具体植物名称，我根据语境推测补充为“植物果实中”，你可根据实际情况修改。
