Rak Agnieszka, Hejmej Anna, Słupecka-Ziemilska Monika, Woliński Jaroslaw, Fiedor Elzbieta, Bilinska Barbara, Gregoraszczuk Ewa Ł
Department of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, Krakow, Poland.
Department of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, Krakow, Poland.
Horm Metab Res. 2017 Sep;49(9):707-715. doi: 10.1055/s-0043-115394. Epub 2017 Jul 31.
This study investigated the effect of a high-fat (HF) diet on protein expression of leptin and its receptor in the gonads of dams and their offspring. Female Wistar rats were fed a HF diet (30% fat) or a standard breeding (BD) diet (5% fat) during pregnancy and lactation. At 21 days of lactation, mothers and both sexes of prepubertal offspring were killed by decapitation. The protein expression of leptin and its receptor was assayed by Western blot and immunohistochemistry in gonadal, periovarian, and epididymal white adipose tissue (WAT). We demonstrated that leptin protein expression in ovary, and both leptin and ObRb expression in periovarian WAT was decreased in HF dams compared with BD animals. Immunohistochemistry showed lower leptin expression in growing antral follicles and corpora lutea of HF dams. Conversely, in both gonads and epididymal WAT of HF offspring, leptin and its receptor were significantly higher expressed compared with BD. Immunolocalization of leptin system in HF offspring gonads showed higher expression in growing and antral follicles of the ovary, seminiferous tubules, and interstitial tissue of testes. In conclusion, high gonadal and gonadal-WAT expression of leptin system was observed in the offspring of dams fed a HF diet during pregnancy and lactation.
本研究调查了高脂（HF）饮食对母鼠及其后代性腺中瘦素及其受体蛋白表达的影响。雌性Wistar大鼠在怀孕和哺乳期间被喂食高脂饮食（30%脂肪）或标准繁殖（BD）饮食（5%脂肪）。在哺乳21天时，通过断头处死母鼠以及青春期前后代的雌雄个体。采用蛋白质印迹法和免疫组织化学法检测性腺、卵巢周围和附睾白色脂肪组织（WAT）中瘦素及其受体的蛋白表达。我们发现，与BD组动物相比，HF组母鼠卵巢中的瘦素蛋白表达以及卵巢周围WAT中的瘦素和ObRb表达均降低。免疫组织化学显示，HF组母鼠生长中的窦状卵泡和黄体中的瘦素表达较低。相反，与BD组相比，HF组后代的性腺和附睾WAT中瘦素及其受体的表达均显著升高。HF组后代性腺中瘦素系统的免疫定位显示，卵巢生长中的卵泡和窦状卵泡、睾丸的生精小管和间质组织中表达较高。总之，在怀孕和哺乳期间喂食高脂饮食的母鼠后代中，观察到性腺和性腺-WAT中瘦素系统的高表达。
