Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Virus Res. 2018 Jan 15;244:321-332. doi: 10.1016/j.virusres.2017.07.020. Epub 2017 Jul 29.
In bacteria and archaea, the clustered regularly interspaced short palindromic repeats (CRISPR) and associated proteins (Cas) confer adaptive immunity against exogenous DNA elements. This CRISPR-Cas system has been turned into an effective tool for editing of eukaryotic DNA genomes. Pathogenic viruses that have a double-stranded DNA (dsDNA) genome or that replicate through a dsDNA intermediate can also be targeted with this DNA editing tool. Here, we review how CRISPR-Cas was used in novel therapeutic approaches against the human immunodeficiency virus type-1 (HIV-1), focusing on approaches that aim to permanently inactivate all virus genomes or to prevent viral persistence in latent reservoirs.
在细菌和古菌中，成簇规律间隔短回文重复序列 (CRISPR) 和相关蛋白 (Cas) 赋予了针对外源性 DNA 元件的适应性免疫。这个 CRISPR-Cas 系统已被转化为编辑真核 DNA 基因组的有效工具。具有双链 DNA (dsDNA) 基因组或通过 dsDNA 中间体复制的致病性病毒也可以用这个 DNA 编辑工具靶向。在这里，我们回顾了 CRISPR-Cas 在针对人类免疫缺陷病毒 1 型 (HIV-1) 的新型治疗方法中的应用，重点介绍了旨在永久失活所有病毒基因组或防止潜伏储库中病毒持续存在的方法。
