CRISPR/Cas9 全景：现状与未来展望。
CRISPR/Cas9 Landscape: Current State and Future Perspectives.
机构信息
Central Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, Russia.
出版信息
Int J Mol Sci. 2023 Nov 8;24(22):16077. doi: 10.3390/ijms242216077.
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a unique genome editing tool that can be easily used in a wide range of applications, including functional genomics, transcriptomics, epigenetics, biotechnology, plant engineering, livestock breeding, gene therapy, diagnostics, and so on. This review is focused on the current CRISPR/Cas9 landscape, e.g., on Cas9 variants with improved properties, on Cas9-derived and fusion proteins, on Cas9 delivery methods, on pre-existing immunity against CRISPR/Cas9 proteins, anti-CRISPR proteins, and their possible roles in CRISPR/Cas9 function improvement. Moreover, this review presents a detailed outline of CRISPR/Cas9-based diagnostics and therapeutic approaches. Finally, the review addresses the future expansion of genome editors' toolbox with Cas9 orthologs and other CRISPR/Cas proteins.
CRISPR（成簇规律间隔短回文重复）/Cas9 是一种独特的基因组编辑工具，可广泛应用于功能基因组学、转录组学、表观遗传学、生物技术、植物工程、家畜育种、基因治疗、诊断等领域。本文综述了 CRISPR/Cas9 的最新进展，例如具有改良特性的 Cas9 变体、Cas9 衍生和融合蛋白、Cas9 的递送方法、针对 CRISPR/Cas9 蛋白的预先存在的免疫反应、抗 CRISPR 蛋白及其在提高 CRISPR/Cas9 功能中的可能作用。此外，本文还详细介绍了基于 CRISPR/Cas9 的诊断和治疗方法。最后，本文探讨了 Cas9 同源物和其他 CRISPR/Cas 蛋白扩展基因组编辑工具盒的未来发展方向。
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