Sidorova Yulia, Shipelin Vladimir, Mazo Vladimir, Zorin Sergey, Petrov Nikita, Kochetkova Alla
Federal State Budgetary Scientific Institution "Federal Research Centre of Nutrition, Biotechnology and Food Safety", Moscow, Russia.
Federal State Budgetary Scientific Institution "Federal Research Centre of Nutrition, Biotechnology and Food Safety", Moscow, Russia.
Nutrition. 2017 Sep;41:107-112. doi: 10.1016/j.nut.2017.04.010. Epub 2017 May 2.
The aim of this study was to examine the hypoglycemic and hypolipidemic effects of Vaccinium myrtillus L. leaf (VLE) and Phaseolus vulgaris L. seed coat (PCE) plant extracts, containing polyphenolic compounds, on carbohydrate and lipid metabolism in Wistar rats with streptozotocin (STZ) and high-fructose diet (HFD)-induced diabetes.
Male Wistar rats with STZ and HFD-induced diabetes were fed with or without VLE or PCE for 50 d. At different intervals, blood glucose and body weight were recorded. Blood samples were used to determine glycated hemoglobin (HbA) and parameters of lipid profile in serum. Additionally, the levels of serum diene conjugates and malondialdehyde were assessed.
Results of HbA and blood glucose level analysis showed the ameliorative effect of VLE on carbohydrate metabolism in diabetic rats. Of the eight diabetic animals treated with bilberry leaf extract, four had normal HbA levels. A significant reduction was observed in VLE group in blood glucose level compared with control diabetic rats (level of hyperglycemia decreased to normal values in 50% of animals versus 0% in the control diabetic group). VLE treatment normalized parameters of lipid metabolism. There was no significant difference from the same parameters in the intact control group. Administration of PCE significantly (P < 0.05) decreased the levels of plasma triacylglycerol (0.81 ± 0.06 mmol/L) and low-density lipoprotein (0.74 ± 0.10 mmol/L) in blood serum compared with the diabetic control group (1.36 ± 0.13 and 1.85 ± 0.30, respectively). Moreover, VLE and PLE showed an antioxidant effect on diene compounds in the blood serum of rats. A significant decrease was found in the plasma diene conjugate levels in PLE group (1.16 ± 0.11 nmol/mL) compared with control diabetic rats (2.09 ± 0.21 nmol/mL; P < 0.05). There was no significant difference from the same parameter in the VLE and intact control groups.
The set of results that was obtained in the present study reveals lipid-lowering and antioxidant action related to the administration of VLE and PCE in Wistar rats with diabetes, induced by STZ, in combination with a high-carbohydrate diet. VLE to a certain extent also reduced hyperglycemia in diabetic animals.
本研究旨在探讨含有多酚类化合物的欧洲越橘叶（VLE）和菜豆种皮（PCE）植物提取物对链脲佐菌素（STZ）和高果糖饮食（HFD）诱导的糖尿病Wistar大鼠碳水化合物和脂质代谢的降血糖和降血脂作用。
将STZ和HFD诱导的糖尿病雄性Wistar大鼠分为两组，分别给予或不给予VLE或PCE，持续喂养50天。在不同时间间隔记录血糖和体重。采集血样测定糖化血红蛋白（HbA）和血清脂质谱参数。此外，评估血清二烯共轭物和丙二醛水平。
HbA和血糖水平分析结果显示VLE对糖尿病大鼠的碳水化合物代谢有改善作用。在用越橘叶提取物治疗的8只糖尿病动物中，4只的HbA水平正常。与对照糖尿病大鼠相比，VLE组血糖水平显著降低（50%的动物高血糖水平降至正常，而对照糖尿病组为0%）。VLE治疗使脂质代谢参数恢复正常。与完整对照组的相同参数相比，无显著差异。与糖尿病对照组相比，给予PCE显著（P < 0.05）降低了血清中血浆三酰甘油（0.81±0.06 mmol/L）和低密度脂蛋白（0.74±0.10 mmol/L）的水平（分别为1.36±0.13和1.85±0.30）。此外，VLE和PLE对大鼠血清中的二烯化合物具有抗氧化作用。与对照糖尿病大鼠相比，PLE组血浆二烯共轭物水平显著降低（1.16±0.11 nmol/mL）（2.09±
