Chowtivannakul Pichaya, Srichaikul Buavaroon, Talubmook Chusri
Department of Biology, Faculty of Science, Mahasarakham University, 44150 Maha Sarakham, Thailand.
Faculty of Public Health, Mahasarakham University, 44150 Maha Sarakham, Thailand.
Pak J Biol Sci. 2016;19(5):211-218. doi: 10.3923/pjbs.2016.211.218.
Antidesma bunius (L.) Spreng has been reported to possess various beneficial medicinal properties. Scientific information about this plant is limited. This study was therefore, designed to determine hypoglycaemic and hypolipidemic effects of ethanol seed extract from A. bunius (ABSE). Antioxidant activity and also acute toxicity were conducted.
The hypoglycaemic and hypolipidemic effects were studied by oral giving ABSE at a dose of 250 mg kg -1 to streptozotocin-induced diabetic rats daily for 6 weeks. Antioxidant activity was studied using DPPH assay. The ABSE at the doses of 500, 1000, 1500 and 2000 mg kg -1 were employed in the acute toxicity study.
The results revealed that ABSE significantly (p<0.05) reduced the blood glucose level and recovered the pathology of hematological values, but significantly (p<0.05) increased the body weight and slightly increased serum insulin of the diabetic rats. However, ABSE recovered pathology of hematological values, but affected renal and hepatic functions in the treated rats by producing an alteration of creatinine, albumin, total protein, BUN and ALP. Interestingly, ABSE increased WBC and HDL, but reduced CHOL, LDL and TG both in normal and diabetic ABSE treated rats. The ABSE possessed relatively low antioxidant activity with IC50 of 2174±14.24 mg mL -1 compared to vitamin C (1.48±0.07 μg mL -1). Fortunately, ABSE did not produce any symptoms of acute toxicity and mortality in the rats.
The ethanol seed extract from A. bunius possesses hypoglycemic and hypolipidemic effects. The ABSE also recovered the pathology of the hematology but may cause renal dysfunction in the diabetic rats. The hypoglycemic and hypolipidemic effects are likely due to its antioxidant and insulin secretion activities.
据报道，五月茶具有多种有益的药用特性。关于这种植物的科学信息有限。因此，本研究旨在确定五月茶乙醇种子提取物（ABSE）的降血糖和降血脂作用，并进行抗氧化活性和急性毒性研究。
通过每天以250mg/kg的剂量口服ABSE，对链脲佐菌素诱导的糖尿病大鼠进行6周，研究其降血糖和降血脂作用。使用DPPH法研究抗氧化活性。在急性毒性研究中使用500、1000、1500和2000mg/kg剂量的ABSE。
结果显示，ABSE显著（p<0.05）降低了血糖水平，恢复了血液学指标异常，但显著（p<0.05）增加了糖尿病大鼠的体重，并轻微增加了血清胰岛素水平。然而，ABSE恢复了血液学指标异常，但通过改变肌酐、白蛋白、总蛋白、尿素氮和碱性磷酸酶，影响了治疗大鼠的肾肝功能值得注意的是，在正常和糖尿病ABSE处理的大鼠中，ABSE均增加了白细胞计数（WBC）和高密度脂蛋白（HDL），但降低了胆固醇（CHOL）、低密度脂蛋白（LDL）和甘油三酯（TG）水平。与维生素C（1.48±0.07μg/mL）相比，ABSE的抗氧化活性相对较低，IC50为2174±14.24mg/mL。幸运的是，ABSE在大鼠中未产生任何急性毒性症状和死亡。
五月茶乙醇种子提取物具有降血糖和降血脂作用。ABSE还恢复了血液学指标异常，但可能导致糖尿病大鼠肾功能障碍。其降血糖和降血脂作用可能归因于其抗氧化和胰岛素分泌活性。
