氟喹诺酮类耐药卡他莫拉菌变异株的逐步选择与分子特征
Molecular Characterization of Fluoroquinolone-Resistant Moraxella catarrhalis Variants Generated by Stepwise Selection.
机构信息
Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan
Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan.
出版信息
Antimicrob Agents Chemother. 2017 Sep 22;61(10). doi: 10.1128/AAC.01336-17. Print 2017 Oct.
Abstract
causes respiratory infections. In this study, fluoroquinolone-resistant strains were selected to evaluate the mechanism of fluoroquinolone resistance. Strains with reduced fluoroquinolone susceptibility were obtained by stepwise selection in levofloxacin, and fluoroquinolone targets and were sequenced. Six novel mutations in GyrA (D84Y, T594dup, and A722dup), GyrB (E479K and D439N), and ParE (Q395R) involved in resistance to fluoroquinolones were revealed.
摘要
导致呼吸道感染。在这项研究中，选择了氟喹诺酮类耐药菌株，以评估氟喹诺酮类耐药的机制。通过逐步选择左氧氟沙星获得了对氟喹诺酮类敏感性降低的菌株，并对氟喹诺酮类靶点和进行了测序。在 GyrA（D84Y、T594dup 和 A722dup）、GyrB（E479K 和 D439N）和 ParE（Q395R）中发现了涉及氟喹诺酮类耐药的六个新突变。
相似文献
1
Molecular Characterization of Fluoroquinolone-Resistant Moraxella catarrhalis Variants Generated by Stepwise Selection.
Antimicrob Agents Chemother. 2017 Sep 22;61(10). doi: 10.1128/AAC.01336-17. Print 2017 Oct.
2
Mutant selection window of four quinolone antibiotics against clinical isolates of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis.
J Infect Chemother. 2018 Feb;24(2):83-87. doi: 10.1016/j.jiac.2017.08.009. Epub 2017 Dec 29.
3
Emergence of fluoroquinolone-resistant Propionibacterium acnes caused by amino acid substitutions of DNA gyrase but not DNA topoisomerase IV.
Anaerobe. 2016 Dec;42:166-171. doi: 10.1016/j.anaerobe.2016.10.012. Epub 2016 Oct 26.
4
Molecular characterization of fluoroquinolone resistance in nontypeable Haemophilus influenzae clinical isolates.
Antimicrob Agents Chemother. 2015 Jan;59(1):461-6. doi: 10.1128/AAC.04005-14. Epub 2014 Nov 10.
5
Role of DNA gyrase and topoisomerase IV mutations in fluoroquinolone resistance of Capnocytophaga spp. clinical isolates and laboratory mutants.
J Antimicrob Chemother. 2017 Aug 1;72(8):2208-2212. doi: 10.1093/jac/dkx119.
6
DNA gyrase and topoisomerase IV mutations in an in vitro fluoroquinolone-resistant Coxiella burnetii strain.
Microb Drug Resist. 2010 Jun;16(2):111-7. doi: 10.1089/mdr.2010.0015.
7
In vitro selection of resistance in Escherichia coli and Klebsiella spp. at in vivo fluoroquinolone concentrations.
BMC Microbiol. 2010 Apr 21;10:119. doi: 10.1186/1471-2180-10-119.
8
Contribution of Type II Topoisomerase Mutations to Fluoroquinolone Resistance in Enterococcus faecium from Japanese Clinical Setting.
Microb Drug Resist. 2018 Jan/Feb;24(1):1-7. doi: 10.1089/mdr.2016.0328. Epub 2017 May 15.
9
In vitro induction and selection of fluoroquinolone-resistant mutants in Elizabethkingia anophelis.
J Microbiol Immunol Infect. 2024 Oct;57(5):822-826. doi: 10.1016/j.jmii.2024.05.011. Epub 2024 Jun 3.
10
Antimicrobial resistance of Moraxella catarrhalis isolates in Taiwan.
J Microbiol Immunol Infect. 2012 Apr;45(2):134-40. doi: 10.1016/j.jmii.2011.09.004. Epub 2011 Dec 9.
引用本文的文献
1
The resistance mechanisms of bacteria against ciprofloxacin and new approaches for enhancing the efficacy of this antibiotic.
Front Public Health. 2022 Dec 21;10:1025633. doi: 10.3389/fpubh.2022.1025633. eCollection 2022.
2
Soft sweep development of resistance in Escherichia coli under fluoroquinolone stress.
J Microbiol. 2019 Dec;57(12):1056-1064. doi: 10.1007/s12275-019-9177-5. Epub 2019 Sep 25.
3
Alternations in DNA gyrase genes in low-level fluoroquinolone-resistant strains isolated in Poland.
Infect Drug Resist. 2018 Aug 6;11:1047-1053. doi: 10.2147/IDR.S162006. eCollection 2018.
本文引用的文献
1
Genetic analysis of a pediatric clinical isolate of Moraxella catarrhalis with resistance to macrolides and quinolones.
J Infect Chemother. 2015 Apr;21(4):308-11. doi: 10.1016/j.jiac.2014.11.002. Epub 2014 Nov 14.
2
Molecular analysis of low-level fluoroquinolone resistance in clinical isolates of Moraxella catarrhalis.
J Med Microbiol. 2014 Aug;63(Pt 8):1066-1070. doi: 10.1099/jmm.0.073734-0. Epub 2014 May 21.
3
The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis.
J Infect Chemother. 2014 Jan;20(1):6-8. doi: 10.1016/j.jiac.2013.06.003. Epub 2013 Nov 12.
4
Antimicrobial susceptibility and mechanisms of high-level macrolide resistance in clinical isolates of Moraxella nonliquefaciens.
J Med Microbiol. 2014 Feb;63(Pt 2):242-247. doi: 10.1099/jmm.0.061788-0. Epub 2013 Nov 6.
5
Molecular mechanism of macrolide-lincosamide resistance in Moraxella catarrhalis.
J Med Microbiol. 2012 Oct;61(Pt 10):1435-1438. doi: 10.1099/jmm.0.046706-0. Epub 2012 Jul 19.
6
Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil.
J Clin Microbiol. 2011 Dec;49(12):4208-12. doi: 10.1128/JCM.01175-11. Epub 2011 Oct 5.
7
bro {beta}-lactamase and antibiotic resistances in a global cross-sectional study of Moraxella catarrhalis from children and adults.
J Antimicrob Chemother. 2010 Jan;65(1):91-7. doi: 10.1093/jac/dkp401.
8
Moraxella catarrhalis, a human respiratory tract pathogen.
Clin Infect Dis. 2009 Jul 1;49(1):124-31. doi: 10.1086/599375.
9
Mechanisms accounting for fluoroquinolone resistance in Escherichia coli clinical isolates.
Antimicrob Agents Chemother. 2009 Jan;53(1):235-41. doi: 10.1128/AAC.00665-08. Epub 2008 Oct 6.
10
Fluoroquinolone resistance in Neisseria meningitidis in Spain.
J Antimicrob Chemother. 2008 Feb;61(2):286-90. doi: 10.1093/jac/dkm452. Epub 2007 Nov 20.
Zotero 插件