Imani Baran Abbas, Cheraghi Saray Habib, Katiraee Farzad
Dept. of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Iran J Parasitol. 2017 Apr-Jun;12(2):243-250.
species are the main causes for fascioliasis with great financial losses and are among the most important food/water-borne parasites worldwide. The basic proceedings such as epidemiology and effective control of fascioliasis rely mainly on precise identification of species. The present study was conducted to determine the species in ruminant fecal samples from East Azerbaijan Province in Iran.
Overall, 2012 fecal samples were collected and processed initially for microscopic examination of eggs in 2014-15. Then, recovered eggs were subjected to molecular identification. A fragment of 618 bp of the 28S rRNA gene pertaining to genus was amplified under PCR. The amplified fragment was restricted by fast digest II enzyme in order to a Restriction Fragment Length Polymorphism.
Based on microscopic examination, 72 samples were infected, from which, 10 and 62 cases pertained to cattle and sheep samples respectively. Based on RFLP, the PCR products restricted by the II restriction enzyme produced 529 bp fragments only. According to the positive controls, all restriction patterns were related to , while no restriction patterns were linked to .
Based on PCR-RFLP, was dominant species in animals of the studied areas and no evidence of was observed. Therefore, further field studies to verify these results are suggested.
片形吸虫是导致片形吸虫病的主要病原体，会造成巨大经济损失，是全球最重要的食源性/水源性寄生虫之一。片形吸虫病的流行病学和有效防控等基础工作主要依赖于对片形吸虫种类的精确鉴定。本研究旨在确定伊朗东阿塞拜疆省反刍动物粪便样本中的片形吸虫种类。
2014 - 2015年共收集了2012份粪便样本并初步处理以进行片形吸虫卵的显微镜检查。然后，对回收的虫卵进行分子鉴定。通过聚合酶链反应（PCR）扩增与片形吸虫属相关的28S核糖体RNA基因的618 bp片段。为了进行限制性片段长度多态性分析，用快速消化酶II对扩增片段进行酶切。
基于显微镜检查，72份样本被感染，其中分别有10例和62例来自牛和羊的样本。基于限制性片段长度多态性分析，用酶II酶切的PCR产物仅产生529 bp的片段。根据阳性对照，所有酶切图谱均与肝片形吸虫相关，而未观察到与大片形吸虫相关的酶切图谱。
基于聚合酶链反应 - 限制性片段长度多态性分析，肝片形吸虫是研究区域动物中的优势种类，未观察到大片形吸虫存在的证据。因此，建议进一步开展实地研究以验证这些结果。
