Sun Zheng, Fang Xiao-Peng, Li Xiao-Yang, Zhou Zhi-Gang
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Appl Biochem Biotechnol. 2018 Feb;184(2):524-537. doi: 10.1007/s12010-017-2564-7. Epub 2017 Jul 31.
Producing biodiesel from microalgae grown in wastewater is environment-friendly and cost-effective. The present study investigated the algae found in wastewater of a local dairy farm for their potential as biodiesel feedstocks. Thirteen native algal strains were isolated. On the basis of morphology and 16S/18S rRNA gene sequences, one strain was identified to be a member of cyanobacteria, while other 12 strains belong to green algae. After screening, two Scenedesmus strains out of the 13 microalgae isolates demonstrated superiority in growth rate, lipid productivity, and sedimentation properties, and therefore were selected for further scale-up outdoor cultivation. Both Scenedesmus strains quickly adapted to the outdoor conditions, exhibiting reasonably good growth and strong anti-contamination capabilities. In flat-plate photobioreactors (PBRs), algal cells accumulated predominantly neutral lipids that accounted for over 60% of total lipids with almost 70% being triacylglycerol. In addition, Scenedesmus obliquus had a high content of monounsaturated fatty acids, of which the amount of oleic acid (C18:1) was up to 27.11%. Based on these findings, the dairy farm wastewater-isolated Scenedesmus strains represent promising sources of low-cost, high-quality oil for biofuel production.
利用养殖在废水中的微藻生产生物柴油既环保又经济高效。本研究调查了当地一家奶牛场废水中发现的藻类作为生物柴油原料的潜力。分离出了13种本地藻类菌株。根据形态学和16S/18S rRNA基因序列，确定其中一个菌株属于蓝藻，而其他12个菌株属于绿藻。经过筛选，13种微藻分离株中的两种栅藻菌株在生长速率、脂质生产率和沉降特性方面表现出优势，因此被选用于进一步扩大规模的室外培养。两种栅藻菌株都能迅速适应室外条件，生长良好且抗污染能力强。在平板光生物反应器（PBR）中，藻细胞主要积累中性脂质，其占总脂质的比例超过60%，其中近70%为三酰甘油。此外，斜生栅藻的单不饱和脂肪酸含量较高，其中油酸（C18:1）的含量高达27.11%。基于这些发现，从奶牛场废水中分离出的栅藻菌株是用于生物燃料生产的低成本、高质量油脂的有前景的来源。
