College of Science, Tibet University, Lhasa City, Tibet Autonomous Region, P. R. China.
Lianyungang Food and Drug Inspection and Testing Center, Lianyungang City, Jiangsu Province, P. R. China.
PLoS One. 2022 Oct 7;17(10):e0275799. doi: 10.1371/journal.pone.0275799. eCollection 2022.
Revegetation of exposed sub-soil, while a desirable recovery strategy, often fails due to extreme soil chemical properties, such as low organic matter and pH levels. Microalgae play a key role in maintaining water quality in the lakes and rivers on the Qinghai-Tibet plateau. Plateau microalgae have extensive application prospects in environmental purification, biotechnology, medicine and cosmetics, food industry, and renewable energy. To identify the high biomass of microalgae present in nature, microalgae with the greatest biomass were screened from natural water samples through filtration, pre-culture, and plate scribing separation. Following identification via 18S rRNA sequencing as for the Desmodesmus sp., we constructed a neighbor-joining phylogenetic tree. The novel Desmodesmus sp. from the Tibetan Yamdrok Lake were identified through polyphasic taxonomy. Simultaneously, the sequence of the experimental samples and the target species were shown different following the identification and analysis of SNP and InDel loci. The light-absorbing properties of plateau Desmodesmus sp. have been investigated previously. The characteristic absorption peak of Desmodesmus sp. on the plateau was measured at 689 nm in the visible spectrum using full wavelength scanning with a UV-Vis spectrophotometer. For Desmodesmus sp. which is prone to settling in the process of amplification culture. By monitoring the change trend of total nitrogen, total phosphorus, pH and electrical conductivity in algae solution system, we determined that the logarithmic growth phase and the best transfer window of Desmodesmus sp. were at 15-20 days. This study can provide basic research methods for the study of microalgae in high altitude areas, and lay a foundation for the later study and application of microalgae.
复育暴露的底土虽然是一种理想的恢复策略，但由于土壤化学性质极端，如有机质和 pH 值低，往往会失败。微藻在维持青藏高原湖泊和河流水质方面发挥着关键作用。高原微藻在环境净化、生物技术、医药和化妆品、食品工业和可再生能源方面具有广泛的应用前景。为了识别自然界中存在的高生物量微藻，通过过滤、预培养和平板划线分离，从天然水样中筛选出生物量最大的微藻。通过 18S rRNA 测序鉴定为束丝藻属后，构建了一个邻接法系统发育树。通过多相分类学鉴定，从西藏羊卓雍错湖中分离出一种新型束丝藻。同时，通过 SNP 和 InDel 位点的鉴定和分析，实验样本的序列与目标物种不同。先前已经研究了高原束丝藻的光吸收特性。使用紫外可见分光光度计对全波长扫描，在可见光谱中测量高原束丝藻的特征吸收峰在 689nm。对于在扩增培养过程中容易沉淀的束丝藻。通过监测藻类溶液系统中总氮、总磷、pH 值和电导率的变化趋势，确定束丝藻的对数生长期和最佳转移窗口在 15-20 天。本研究可为高海拔地区微藻的研究提供基础研究方法，为微藻的后期研究和应用奠定基础。
