孕激素对脂多糖诱导的人宫颈成纤维细胞表达变化的作用多样性，取决于炎症和治疗时间。
Diversity of progesterone action on lipopolysaccharide-induced expression changes in cultured human cervical fibroblasts according to inflammation and treatment timing.
机构信息
Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.
Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan.
出版信息
Am J Reprod Immunol. 2017 Nov;78(5). doi: 10.1111/aji.12731. Epub 2017 Aug 1.
PROBLEM
The effectiveness of progesterone (P4) treatment for preventing preterm births is unclear. Its effects on the uterine cervix were tested using cultured human uterine cervical fibroblasts (UCFs).
METHOD OF STUDY
UCFs were incubated with lipopolysaccharide (LPS) in the presence or absence of P4 under various conditions. mRNA was subjected to PCR arrays and real-time RT-PCR to assess IL-6, IL-8, IL-1beta, PTGS2, MMP-1, and CXCL10 expression.
RESULTS
When exposed to a high-LPS concentration (2.0 μg/mL), expression of these genes was not suppressed by simultaneous P4 (1.0 μmol/L) treatment, but it was significantly inhibited when P4 was administered 1 hour prior to LPS, with the exception of the chemokines IL-8 and CXCL10. Expression of all genes was restricted by P4 under low-level LPS (0.2 μg/mL) stimulation, especially when administered prior to LPS treatment.
CONCLUSION
These data suggest that early or prophylactic P4 administration is an effective and important measure for reducing preterm birth risk.
问题
孕激素（P4）治疗预防早产的效果尚不清楚。本研究使用培养的人子宫颈成纤维细胞（UCFs）检测 P4 对子宫颈的作用。
方法
在不同条件下，用脂多糖（LPS）孵育 UCFs，同时或不使用 P4。采用 PCR 芯片和实时 RT-PCR 检测 IL-6、IL-8、IL-1β、PTGS2、MMP-1 和 CXCL10 的表达。
结果
当暴露于高 LPS 浓度（2.0 μg/mL）时，同时给予 P4（1.0 μmol/L）治疗并不能抑制这些基因的表达，但当 P4 在 LPS 之前 1 小时给药时，除趋化因子 IL-8 和 CXCL10 外，其表达明显受到抑制。在低水平 LPS（0.2 μg/mL）刺激下，所有基因的表达均受到 P4 的限制，尤其是在 LPS 处理之前给药时。
结论
这些数据表明，早期或预防性 P4 给药是降低早产风险的有效且重要的措施。
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