Department of Craniofacial Surgery, University Hospital Schleswig-Holstein , Kiel, Germany .
Tissue Eng Part C Methods. 2017 Dec;23(12):842-849. doi: 10.1089/ten.TEC.2017.0086. Epub 2017 Sep 20.
The reconstruction of defects in the mandible are still challenging. Despite several adequate microvascular bone reconstruction techniques, there is a need for ectopic bone endocultivation without drawbacks by donor-site morbidity. The omentum majus is described as a good vascularized fleece with undifferentiated cells with potential for bone culturing. In the omentum majus of six rabbits, two hydroxyapatite blocks were incorporated for 12 weeks each. The blocks were prepared with recombinant human bone morphogenetic protein-2 (rhBMP-2) or VEGF + rhBMP-2 and wrapped into the omentum. For ectopic bone endocultivation observation computed tomography (CT) scans were performed, and fluorescence markers were applied. After harvesting the block, histological sections were performed with hematoxylin and eosin and toluidine blue staining. In the CT scans, the Hounsfield units of the blocks increased within the trail. In some sections, new bone formation was observed within the hydroxyapatite blocks, however, the histological staining showed soft-tissue invasion only, no gross bone formation was observed. The ectopic bone endocultivation in the omentum majus is technically a good approach. An adequate mixture of osteoinductive proteins is still missing.
下颌骨缺损的重建仍然具有挑战性。尽管有几种适当的微血管骨重建技术，但仍需要通过供体部位发病率来进行无缺点的异位骨内培养。大网膜被描述为一种具有未分化细胞的良好血管化绒毛，具有骨培养的潜力。在六只兔子的大网膜中，每只各植入两块羟基磷灰石块，持续 12 周。这些块用重组人骨形态发生蛋白-2(rhBMP-2)或 VEGF+rhBMP-2 制备，并包裹在大网膜中。为了进行异位骨内培养观察，进行了计算机断层扫描(CT)扫描，并应用了荧光标记物。在取出块后，进行了苏木精和伊红以及甲苯胺蓝染色的组织学切片。在 CT 扫描中，块内的 Hounsfield 单位增加。在一些切片中，在羟基磷灰石块内观察到新骨形成，然而，组织学染色仅显示软组织浸润，未观察到明显的骨形成。大网膜内的异位骨内培养在技术上是一种很好的方法。仍然缺乏足够的成骨诱导蛋白混合物。
