Department of Oral & Maxillofacial Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.
Regen Med. 2020 Nov;15(11):2297-2309. doi: 10.2217/rme-2020-0115. Epub 2020 Dec 23.
This study aimed to evaluate two different vascularized bone flap scaffolds and the impact of two barrier membranes for the reconstruction of critical-size bone defects. 3D-printed scaffolds of biodegradable calcium phosphate and bioinert titanium were loaded with rhBMP-2 bone marrow aspirate, wrapped by a collagen membrane or a periosteum transplant and implanted into the greater omentum of miniature pigs. Histological evaluation demonstrated significant bone formation within the first 8 weeks in both scaffolds. The periosteum transplant led to enhanced bone formation and a homogenous distribution in the scaffolds. The omentum tissue grew out a robust vascular supply. Endocultivation using 3D-printed scaffolds in the greater omentum is a very promising approach in defect-specific bone tissue regeneration.
本研究旨在评估两种不同的血管化骨瓣支架以及两种屏障膜对临界尺寸骨缺损重建的影响。将可生物降解的磷酸钙和生物惰性钛的 3D 打印支架装载 rhBMP-2 骨髓抽吸物，用胶原膜或骨膜移植包裹，并植入小型猪的大网膜中。组织学评估表明，在最初的 8 周内，两种支架内均有明显的骨形成。骨膜移植导致骨形成增加，支架内分布均匀。大网膜组织生长出丰富的血管供应。在大网膜中使用 3D 打印支架进行内生培养是一种很有前途的针对特定缺陷的骨组织再生方法。
