Soto-Quintero Albanelly, Romo-Uribe Ángel, Bermúdez-Morales Víctor H, Quijada-Garrido Isabel, Guarrotxena Nekane
Centro de Investigación en Ingeniería y Ciencias Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico.
Research & Development, Advanced Science & Technology Division, Johnson & Johnson Vision, Jacksonville, FL 32256, USA.
Nanomaterials (Basel). 2017 Aug 1;7(8):209. doi: 10.3390/nano7080209.
This study underscores the development of Ag hydrogel nanocomposites, as smart substrates for antibacterial uses, via innovative in situ reactive and reduction pathways. To this end, two different synthetic strategies were used. Firstly thiol-acrylate (PSA) based hydrogels were attained via thiol-ene and radical polymerization of polyethylene glycol (PEG) and polycaprolactone (PCL). As a second approach, polyurethane (PU) based hydrogels were achieved by condensation polymerization from diisocyanates and PCL and PEG diols. In fact, these syntheses rendered active three-dimensional (3D) hydrogel matrices which were used as nanoreactors for in situ reduction of AgNO₃ to silver nanoparticles. A redox chemistry of stannous catalyst in PU hydrogel yielded spherical AgNPs formation, even at 4 °C in the absence of external reductant; and an appropriate thiol-functionalized polymeric network promoted spherical AgNPs well dispersed through PSA hydrogel network, after heating up the swollen hydrogel at 103 °C in the presence of citrate-reductant. Optical and swelling behaviors of both series of hydrogel nanocomposites were investigated as key factors involved in their antimicrobial efficacy over time. Lastly, in vitro antibacterial activity of Ag loaded hydrogels exposed to and strains indicated a noticeable sustained inhibitory effect, especially for Ag-PU hydrogel nanocomposites with bacterial inhibition growth capabilities up to 120 h cultivation.
本研究强调了通过创新的原位反应和还原途径开发银水凝胶纳米复合材料作为抗菌用途的智能基材。为此，使用了两种不同的合成策略。首先，通过聚乙二醇（PEG）和聚己内酯（PCL）的硫醇-烯和自由基聚合获得基于硫醇-丙烯酸酯（PSA）的水凝胶。作为第二种方法，基于聚氨酯（PU）的水凝胶通过二异氰酸酯与PCL和PEG二醇的缩聚反应制备。事实上，这些合成产生了活性三维（3D）水凝胶基质，用作将AgNO₃原位还原为银纳米颗粒的纳米反应器。PU水凝胶中亚锡催化剂的氧化还原化学即使在4°C且无外部还原剂的情况下也能产生球形AgNP；在柠檬酸盐还原剂存在下将溶胀的水凝胶在103°C加热后，合适的硫醇官能化聚合物网络促进了球形AgNP在PSA水凝胶网络中良好分散。研究了这两种系列水凝胶纳米复合材料的光学和溶胀行为，作为随时间推移其抗菌功效的关键因素。最后，负载银的水凝胶对 和 菌株的体外抗菌活性表明了显著的持续抑制作用，特别是对于具有长达120小时培养细菌抑制生长能力的Ag-PU水凝胶纳米复合材料。
