a Centre of Biological Engineering (CEB) , University of Minho , Braga , Portugal.
b International Joint Research Laboratory for Textile and Fiber Bioprocesses , Jiangnan University , Wuxi , China.
Crit Rev Biotechnol. 2018 May;38(3):335-350. doi: 10.1080/07388551.2017.1355294. Epub 2017 Aug 1.
Enzymes are efficient catalysts designed by nature to work in physiological environments of living systems. The best operational conditions to access and convert substrates at the industrial level are different from nature and normally extreme. Strategies to isolate enzymes from extremophiles can redefine new operational conditions, however not always solving all industrial requirements. The stability of enzymes is therefore a key issue on the implementation of the catalysts in industrial processes which require the use of extreme environments that can undergo enzyme instability. Strategies for enzyme stabilization have been exhaustively reviewed, however they lack a practical approach. This review intends to compile and describe the most used approaches for enzyme stabilization highlighting case studies in a practical point of view.
酶是自然界设计的高效催化剂，旨在在生命系统的生理环境中发挥作用。在工业层面上获取和转化底物的最佳操作条件与自然界不同，通常是极端的。从极端微生物中分离酶的策略可以重新定义新的操作条件，但并不总能满足所有工业需求。因此，酶的稳定性是在需要使用可能导致酶失稳的极端环境的工业过程中实施催化剂的关键问题。酶稳定剂的策略已经被广泛综述，但缺乏实际的方法。本综述旨在收集和描述最常用的酶稳定化方法，并突出实际角度的案例研究。
