Redondo Leandro M, Redondo Enzo A, Dailoff Gabriela C, Leiva Carlos L, Díaz-Carrasco Juan M, Bruzzone Octavio A, Cangelosi Adriana, Geoghegan Patricia, Fernandez-Miyakawa Mariano E
Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Calle Las Cabañas y Los Reseros s/n, Casilla de Correo 25, 1712 Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia 1917, 1033 Ciudad Autónoma de Buenos Aires, Argentina.
EEA Bariloche, Instituto Nacional de Tecnología Agropecuaria, Modesta Victoria 4450, 8400 Bariloche, Río Negro, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia 1917, 1033 Ciudad Autónoma de Buenos Aires, Argentina.
Anaerobe. 2017 Dec;48:83-88. doi: 10.1016/j.anaerobe.2017.07.007. Epub 2017 Jul 29.
Iota toxin is a binary toxin solely produced by Clostridium perfringens type E strains, and is structurally related to CDT from C. difficile and CST from C. spiroforme. As type E causes hemorrhagic enteritis in cattle, it is usually assumed that associated diseases are mediated by iota toxin, although evidence in this regard has not been provided. In the present report, iota toxin intestinal effects were evaluated in vivo using a mouse model. Histological damage was observed in ileal loops treated with purified iota toxin after 4 h of incubation. Luminal iota toxin induced fluid accumulation in the small intestine in a dose dependent manner, as determined by the enteropooling and the intestinal loop assays. None of these changes were observed in the large intestine. These results suggest that C. perfringens iota toxin alters intestinal permeability, predominantly by inducing necrosis and degenerative changes in the mucosal epithelium of the small intestine, as well as changes in intestinal motility. The obtained results suggest a central role for iota toxin in the pathogenesis of C. perfringens type E hemorrhagic enteritis, and contribute to remark the importance of clostridial binary toxins in digestive diseases.
埃塔毒素是一种仅由产气荚膜梭菌E型菌株产生的二元毒素，在结构上与艰难梭菌的细胞毒素扩张毒素（CDT）和螺旋体梭菌的梭菌二元毒素（CST）相关。由于E型菌株会导致牛的出血性肠炎，通常认为相关疾病是由埃塔毒素介导的，尽管尚未提供这方面的证据。在本报告中，使用小鼠模型在体内评估了埃塔毒素对肠道的影响。孵育4小时后，在用纯化的埃塔毒素处理的回肠袢中观察到组织学损伤。通过肠内积液和肠袢试验确定，肠腔内的埃塔毒素以剂量依赖性方式诱导小肠内液体蓄积。在大肠中未观察到这些变化。这些结果表明，产气荚膜梭菌埃塔毒素主要通过诱导小肠黏膜上皮的坏死和退行性变化以及肠道运动的改变来改变肠道通透性。所得结果表明埃塔毒素在产气荚膜梭菌E型出血性肠炎的发病机制中起核心作用，并有助于强调梭菌二元毒素在消化系统疾病中的重要性。
