Campagne Cécil, Pons Stéphanie, Esquerre Diane, Estellé Jordi, Bourneuf Emmanuelle, Maskos Uwe, Egidy Giorgia
INRA, UMR955 Génétique Fonctionnelle et Médicale, Ecole Nationale Vétérinaire d'Alfort, F-94704 Maisons-Alfort, France.
Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, UMR955 Génétique Fonctionnelle et Médicale, F-94704 Maisons-Alfort, France.
Data Brief. 2017 Jul 11;14:32-34. doi: 10.1016/j.dib.2017.07.006. eCollection 2017 Oct.
The transcriptome sequencing of melanoma cells from two mouse models differing in the expression level of the scaffold protein Receptor for activated C kinase (RACK1) are presented. Primary melanoma cells were harvested from mice, with or without the transgene. Cells were cultured and infected with scramble shRNA or -targeting shRNA on technical triplicates of viral infection. Libraries were prepared by selecting polyadenylated mRNAs and RNA Sequencing (RNASeq) was performed. Samples are described in the SRA portal (SRP096162) and FASTQ files have been deposited in Sequence Read Archive (accession numbers: SRR5150106 to SRR5150117). The interpretation of these data is presented in the following research article: "RACK1 cooperates with NRAS to promote melanoma " (Campagne et al., 2017, doi: 10.1016/j.cellsig.2017.03.015) [1].
本文展示了来自两种小鼠模型的黑色素瘤细胞的转录组测序结果，这两种模型中支架蛋白活化C激酶受体（RACK1）的表达水平不同。从有或无转基因的小鼠中收获原发性黑色素瘤细胞。细胞进行培养，并在病毒感染的技术重复中用乱序短发夹RNA（shRNA）或靶向shRNA进行感染。通过选择聚腺苷酸化的mRNA制备文库，并进行RNA测序（RNA Seq）。样本在SRA数据库（SRP096162）中有所描述，FASTQ文件已存入序列读取存档库（登录号：SRR5150106至SRR5150117）。这些数据的解读见以下研究文章：《RACK1与NRAS协同促进黑色素瘤》（坎帕涅等人，2017年，doi:10.1016/j.cellsig.2017.03.015）[1]。
