Hughes C S, Longo E, Phillips-Jones M K, Hussain R
Diamond Light Source, Harwell Research & Innovation Campus, Chilton, Didcot OX11 0DE, United Kingdom.
Membranes, Membrane Proteins & Peptides Research Group, School of Pharmacy & Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, United Kingdom.
Data Brief. 2017 Jul 13;14:41-47. doi: 10.1016/j.dib.2017.07.012. eCollection 2017 Oct.
This data article presents the results from quality control experiments including N-terminal sequencing, SEC-MALS and Mass Spectrometry for purified VanS used in experiments described in (Hughes et al., 2017) [1]; in addition to ligand interaction measurements and thermal melting curves of VanS in the presence of screened ligands from circular dichroism measurements as well as UV-vis absorbance spectra for the binding interaction of VanS in the presence of screened ligands.
本数据文章展示了质量控制实验的结果,这些实验包括对(休斯等人,2017年)[1]所述实验中使用的纯化VanS进行N端测序、尺寸排阻色谱-多角度激光光散射联用(SEC-MALS)和质谱分析;此外还包括配体相互作用测量以及在圆二色性测量中筛选出的配体存在下VanS的热熔解曲线,以及在筛选出的配体存在下VanS结合相互作用的紫外可见吸收光谱。