大鼠肾脏γ-谷氨酰转移酶对谷胱甘肽和谷氨酰胺的同时水解作用。
The simultaneous hydrolysis of glutathione and glutamine by rat kidney gamma-glutamyltransferase.
作者信息
Cook N D, Peters T J
出版信息
Biochim Biophys Acta. 1986 Oct 29;884(1):207-10. doi: 10.1016/0304-4165(86)90245-x.
The simultaneous hydrolysis of L-glutamine and glutathione by rat kidney gamma-glutamyltransferase has been studied. At concentrations of the two substrates giving rates approximating to Vmax, it is shown that glutamine hydrolysis predominates. Under saturating conditions for both substrates, 65% of the glutamic acid produced is derived from glutamine. Using [14C]glutamine it is also shown that at physiological plasma concentrations of glutamine (in excess of 400 microM) and glutathione (20 microM) glutamine hydrolysis is the predominating reaction. We therefore suggest that both glutamine and glutathione are important in vivo donors for gamma-glutamyltransferase.
对大鼠肾脏γ-谷氨酰转移酶同时水解L-谷氨酰胺和谷胱甘肽的情况进行了研究。在两种底物浓度达到近似Vmax的反应速率时，结果表明谷氨酰胺水解占主导。在两种底物均饱和的条件下，所产生的谷氨酸中有65%来自谷氨酰胺。使用[14C]谷氨酰胺还表明，在谷氨酰胺（超过400微摩尔）和谷胱甘肽（20微摩尔）的生理血浆浓度下，谷氨酰胺水解是主要反应。因此，我们认为谷氨酰胺和谷胱甘肽在体内都是γ-谷氨酰转移酶的重要供体。
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