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鸡源 H5N1 禽流感病毒的系统发育、致病性和传播。

Phylogeny, Pathogenicity, and Transmission of H5N1 Avian Influenza Viruses in Chickens.

机构信息

Animal Infectious Diseases Laboratory, College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China.

National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and ControlGuangzhou, China.

出版信息

Front Cell Infect Microbiol. 2017 Jul 19;7:328. doi: 10.3389/fcimb.2017.00328. eCollection 2017.

DOI:10.3389/fcimb.2017.00328
PMID:28770175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5515836/
Abstract

We analyzed five H5N1 avian influenza viruses (AIVs) isolated from different birds in 2012 in China. Based on whole-genome sequences, we divided the viruses into four genotypes. The DKE26, GSE43, and DKE53 viruses belonged to Genotypes 1-3, respectively. The CKE93 and CKE96 viruses were classified into Genotype 4. Genotypes 1-3 correspond to the viruses containing the HA gene of clade 2.3.2, and Genotype 4 is the virus that bears the HA gene of clade 7.2. To better understand the pathogenicity and transmission of the viruses, we infected chickens with 10 EID/0.1 ml GSE43 (clade 2.3.2) or CKE93 (clade 7.2) virus. Our results revealed that 6 of 7 specific-pathogen-free (SPF) chickens inoculated with GSE43 virus were dead before 7-day post-infection, but all the SPF chickens inoculated with CKE93 virus survived the infection. Both the GSE43 and CKE93 viruses replicated systemically in chickens. The virus titers of GSE43 virus in tested organs were obviously higher than those of CKE93 virus. Our results revealed that the pathogenicity and replication of GSE43 in chickens was much higher than those of CKE93. The GSE43 virus could transmit between chickens, but the CKE93 could not transmit between chickens by naïve contact. Therefore, different clades of H5N1 AIVs possessed variable pathogenicities and transmission abilities among chickens. Our study contributes to knowledge of pathogenic variations of prevalent H5N1 viruses.

摘要

我们分析了 2012 年在中国从不同鸟类中分离的五株 H5N1 禽流感病毒（AIVs）。基于全基因组序列，我们将这些病毒分为四个基因型。DKE26、GSE43 和 DKE53 病毒分别属于基因型 1-3。CKE93 和 CKE96 病毒被归类为基因型 4。基因型 1-3 对应于含有 clade 2.3.2 的 HA 基因的病毒，基因型 4 是携带 clade 7.2 的 HA 基因的病毒。为了更好地了解病毒的致病性和传播性，我们用 10 EID/0.1 ml 的 GSE43（clade 2.3.2）或 CKE93（clade 7.2）病毒感染鸡。我们的结果表明，用 GSE43 病毒感染的 7 只无特定病原体（SPF）鸡中有 6 只在感染后 7 天前死亡，但所有用 CKE93 病毒感染的 SPF 鸡都存活下来。GSE43 和 CKE93 病毒在鸡体内均能系统复制。GSE43 病毒在检测器官中的病毒滴度明显高于 CKE93 病毒。我们的结果表明，GSE43 在鸡中的致病性和复制能力远高于 CKE93。GSE43 病毒可以在鸡之间传播，但 CKE93 病毒不能通过未经处理的接触在鸡之间传播。因此，不同 clades 的 H5N1 AIVs 在鸡之间具有不同的致病性和传播能力。我们的研究有助于了解流行的 H5N1 病毒的致病性变化。

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