Nepom G T, Seyfried C E, Holbeck S L, Wilske K R, Nepom B S
Lancet. 1986 Nov 1;2(8514):1002-5. doi: 10.1016/s0140-6736(86)92614-0.
Genes of the major histocompatibility complex, HLA, are associated with susceptibility to rheumatoid arthritis (RA), but the aetiology of this chronic inflammatory disease is not known. Synthetic oligonucleotide DNA probes were constructed to distinguish between two closely related but distinct alleles encoding the HLA-DR4 specificity in patients with RA. With these allele-specific oligonucleotide probes an uncommon DR4 genetic variant, Dw14, was identified in 6 of 7 RA patients homozygous for HLA-DR4. This allele may play an important part in susceptibility to RA.
主要组织相容性复合体（MHC）的基因，即人类白细胞抗原（HLA），与类风湿性关节炎（RA）的易感性相关，但这种慢性炎症性疾病的病因尚不清楚。构建了合成寡核苷酸DNA探针，以区分编码RA患者中HLA - DR4特异性的两个密切相关但不同的等位基因。利用这些等位基因特异性寡核苷酸探针，在7名HLA - DR4纯合的RA患者中，有6名被鉴定出一种罕见的DR4基因变体Dw14。该等位基因可能在RA易感性中起重要作用。
