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在透明质酸-明胶复合支架中，较高比例的透明质酸可增强人骨髓间充质干细胞的软骨分化。

Higher Ratios of Hyaluronic Acid Enhance Chondrogenic Differentiation of Human MSCs in a Hyaluronic Acid-Gelatin Composite Scaffold.

作者信息

Pfeifer Christian G, Berner Arne, Koch Matthias, Krutsch Werner, Kujat Richard, Angele Peter, Nerlich Michael, Zellner Johannes

机构信息

Department of Trauma Surgery, University Medical CentreRegensburg, Franz-Josef-Strauss-Allee 11, Regensburg 93053, Germany.

Sporthopaedicum, Straubing/Regensburg, Hildegard-von-Bingen-Str. 1, Regensburg 93053, Germany.

出版信息

Materials (Basel). 2016 May 17;9(5):381. doi: 10.3390/ma9050381.

DOI:10.3390/ma9050381
PMID:28773501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5503045/
Abstract

UNLABELLED

Mesenchymal stem cells (MSCs) seeded on specific carrier materials are a promising source for the repair of traumatic cartilage injuries. The best supportive carrier material has not yet been determined. As natural components of cartilage's extracellular matrix, hyaluronic acid and collagen are the focus of biomaterial research. In order to optimize chondrogenic support, we investigated three different scaffold compositions of a hyaluronic acid (HA)-gelatin based biomaterial.

METHODS

Human MSCs (hMSCs) were seeded under vacuum on composite scaffolds of three different HA-gelatin ratios and cultured in chondrogenic medium for 21 days. Cell-scaffold constructs were assessed at different time points for cell viability, gene expression patterns, production of cartilage-specific extracellular matrix (ECM) and for (immuno-)histological appearance. The intrinsic transforming growth factor beta (TGF-beta) uptake of empty scaffolds was evaluated by determination of the TGF-beta concentrations in the medium over time.

RESULTS

No significant differences were found for cell seeding densities and cell viability. hMSCs seeded on scaffolds with higher ratios of HA showed better cartilage-like differentiation in all evaluated parameters. TGF-beta uptake did not differ between empty scaffolds.

CONCLUSION

Higher ratios of HA support the chondrogenic differentiation of hMSCs seeded on a HA-gelatin composite scaffold.

摘要

未标记

接种在特定载体材料上的间充质干细胞（MSC）是修复创伤性软骨损伤的一个有前景的来源。目前尚未确定最佳的支持性载体材料。作为软骨细胞外基质的天然成分，透明质酸和胶原蛋白是生物材料研究的重点。为了优化软骨生成支持，我们研究了基于透明质酸（HA）-明胶的生物材料的三种不同支架组成。

方法

将人MSC（hMSC）在真空条件下接种到三种不同HA-明胶比例的复合支架上，并在软骨生成培养基中培养21天。在不同时间点评估细胞-支架构建体的细胞活力、基因表达模式、软骨特异性细胞外基质（ECM）的产生以及（免疫）组织学外观。通过测定培养基中随时间变化的TGF-β浓度来评估空支架对内在转化生长因子β（TGF-β）的摄取。

结果

细胞接种密度和细胞活力未发现显著差异。接种在HA比例较高的支架上的hMSC在所有评估参数中显示出更好的软骨样分化。空支架之间的TGF-β摄取没有差异。

结论

较高比例的HA支持接种在HA-明胶复合支架上的hMSC的软骨生成分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/7edd1e31646e/materials-09-00381-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/7b8cf9ccb132/materials-09-00381-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/07e620288909/materials-09-00381-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/1a98085f5103/materials-09-00381-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/17214ebe4639/materials-09-00381-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/7edd1e31646e/materials-09-00381-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/7b8cf9ccb132/materials-09-00381-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/3873bbaa1dd0/materials-09-00381-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/f5eccf63db91/materials-09-00381-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/ab4e6b027af5/materials-09-00381-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/07e620288909/materials-09-00381-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/1a98085f5103/materials-09-00381-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/17214ebe4639/materials-09-00381-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6f1/5503045/7edd1e31646e/materials-09-00381-g007.jpg

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