Baldino F, Wolfson B, Heinemann U, Gutnick M J
Neurosci Lett. 1986 Sep 25;70(1):101-5. doi: 10.1016/0304-3940(86)90445-3.
We investigated the actions of a specific N-methyl-D-aspartate (NMDA) antagonist, 2-amino-5-phosphonovaleric acid (2-APV), on bicuculline-induced epileptogenesis in organotypic explant cultures from neonatal rat neocortex. Explants were maintained in roller tubes for 3-5 weeks. The late, plateau phase of the intracellularly recorded paroxysmal depolarization shift was sensitive to both intracellularly injected hyperpolarizing currents and 2-APV, suggesting that this component is generated by a voltage-dependent, regenerative process that is mediated by activation of NMDA receptors. The results support the hypothesis that NMDA receptors play an important role in the generation of epileptiform activity by localized circuits of neocortical neurons.
我们研究了一种特定的N-甲基-D-天冬氨酸（NMDA）拮抗剂2-氨基-5-磷酸戊酸（2-APV）对新生大鼠新皮质器官型外植体培养物中荷包牡丹碱诱导的癫痫发生的作用。外植体在滚瓶中培养3至5周。细胞内记录的阵发性去极化偏移的晚期平台期对细胞内注入的超极化电流和2-APV均敏感，这表明该成分是由NMDA受体激活介导的电压依赖性再生过程产生的。结果支持以下假设，即NMDA受体在新皮质神经元局部回路产生癫痫样活动中起重要作用。
