Department of Neuroscience & Regenerative Medicine, USA.
Department of Mathematics, Logistical Engineering University, Chongqing, China.
Mol Cell Endocrinol. 2018 Aug 15;471:97-104. doi: 10.1016/j.mce.2017.07.037. Epub 2017 Jul 31.
Peroxisome proliferator-activated receptor gamma (PPARγ) is a key regulator of adipogenesis. It is also a central player in energy metabolism, inflammation and immunity. As an important nuclear transcription factor, PPARγ can regulate the expression and function of genes or biological processes directly or indirectly via association with other factors and thus modulate their activities. To better understand the impact of PPARγ on the global gene expression profile, we evaluated the bioinformatic data, which revealed the changes that occurred in genes and their pathways in the absence of PPARγ. In brief, we performed RNA deep sequencing (RNA-Seq) analysis using RNA samples isolated from multipotent mesenchymal stromal cells (MSCs) of PPARγ knockout and wild type control mice. The RNA-Seq data sets were then subjected to bioinformatic analyses from various angles to better reveal the breadth of PPARγ function in different biological processes. Our results reveal novel genes and networks modulated by PPARγ and provides new insights into our understanding of the physiologic and pathophysiologic role this nuclear receptor plays in health and disease.
过氧化物酶体增殖物激活受体 γ (PPARγ) 是脂肪生成的关键调节因子。它也是能量代谢、炎症和免疫的核心参与者。作为一种重要的核转录因子，PPARγ 可以通过与其他因子的结合直接或间接调节基因或生物过程的表达和功能，从而调节它们的活性。为了更好地了解 PPARγ 对全球基因表达谱的影响，我们评估了生物信息学数据，这些数据揭示了在缺乏 PPARγ 的情况下基因及其途径发生的变化。简而言之，我们使用来自 PPARγ 敲除和野生型对照小鼠多能间充质基质细胞 (MSC) 的 RNA 样本进行了 RNA 深度测序 (RNA-Seq) 分析。然后，我们从不同角度对 RNA-Seq 数据集进行了生物信息学分析，以更好地揭示 PPARγ 在不同生物学过程中的功能广度。我们的结果揭示了受 PPARγ 调节的新基因和网络，并为我们理解该核受体在健康和疾病中的生理和病理生理作用提供了新的见解。
