Wegehaupt Abby K, Roufs Ellen K, Hewitt Cory R, Killian Marisela L, Gorbatenko Oxana, Anderson Cynthia M, Killian M Scott
Department of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, United States.
Flow Cytometry Core Facility, Sanford Research, Sioux Falls, SD 57104, United States.
Biologicals. 2017 Sep;49:15-22. doi: 10.1016/j.biologicals.2017.07.006. Epub 2017 Jul 31.
Leukocytes, or white blood cells, are used for a variety of investigational purposes and they offer advantages over laboratory-adapted cell lines. Leukocytes that are typically discarded by blood banks during the collection of red blood cells, platelets, and plasma can often be obtained for research use. However, the available leukocytes are frequently contained within a blood filtration device, such as the Terumo LR Express (TLRE) filter. In this study, procedures were evaluated for the ability to elute viable leukocytes from TLRE filters. The recovered leukocytes were assessed for composition, growth, and functionality. The large majority (>70%) of leukocytes were eluted with a single reverse-elution procedure and the recovered cells contained representative populations of the major leukocyte subsets. Purified T cells exhibited diverse T cell receptor repertoires, characteristic growth upon mitogen stimulation, and CD4 T cells were able to support HIV-1 propagation. Purified monocytes were able to be differentiated into phenotypically characteristic populations of macrophages and dendritic cells. Overall, TLRE filters offer an attractive source of primary human cells for research and possibly clinical purposes.
白细胞，即白血球，可用于多种研究目的，且相较于实验室适应的细胞系具有优势。在采集红细胞、血小板和血浆过程中通常被血库丢弃的白细胞，往往可获取用于研究。然而，可得的白细胞常存在于血液过滤装置中，如泰尔茂LR Express（TLRE）过滤器。在本研究中，对从TLRE过滤器洗脱活白细胞的能力进行了评估。对回收的白细胞进行了成分、生长和功能评估。绝大多数（>70%）白细胞通过单次反向洗脱程序被洗脱，回收的细胞包含主要白细胞亚群的代表性群体。纯化的T细胞表现出多样的T细胞受体库、在有丝分裂原刺激下的特征性生长，且CD4 T细胞能够支持HIV-1增殖。纯化的单核细胞能够分化为具有表型特征的巨噬细胞和树突状细胞群体。总体而言，TLRE过滤器为研究及可能的临床目的提供了一种有吸引力的原代人类细胞来源。
