血小板释放生长因子和骨增量材料（Bio-Oss）对乙醇损伤成骨细胞的保护作用。
The protective effect of platelet released growth factors and bone augmentation (Bio-Oss) on ethanol impaired osteoblasts.
作者信息
Sönmez Tolga Taha, Bayer Andreas, Cremer Tillman, Hock Jennifer Vanessa Phi, Lethaus Bernd, Kweider Nisreen, Wruck Christoph Jan, Drescher Wolf, Jahr Holger, Lippross Sebastian, Pufe Thomas, Tohidnezhad Mersedeh
机构信息
Department of Oral and Maxillofacial Surgery, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074 Aachen, Germany; Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.
Department of Heart and Vascular Surgery, University Hospital of Schleswig-Holstein, Kiel, Germany.
出版信息
Ann Anat. 2017 Nov;214:36-42. doi: 10.1016/j.aanat.2017.07.002. Epub 2017 Jul 31.
BACKGROUND
Chronic alcohol consumption is a known limiting factor for bone healing. One promising strategy to improve bone augmentation techniques with Bio-Oss in oral and maxillofacial surgery might be the supportive application of platelet-concentrated biomaterials as platelet-released growth factor (PRGF). To address this matter, we performed an in vitro study investigating the protective effects of PRGF and Bio-Oss in ethanol (EtOH) treated osteoblasts.
METHODS
The SAOS-2 osteosarcoma cell line, with and without EtOH pretreatment was used. The cell viability, proliferation and alkali phosphatase activity (ALP) after application of 0%, 5% and 10% PRGF and Bio-Oss were assessed.
RESULTS
The application of PRGF and Bio-Oss in EtOH impaired osteoblasts showed a significant beneficial influence increasing the viability of the osteoblasts in cell culture. The synergistic effect of Bio-Oss and 5% PRGF on the proliferation of osteoblasts was also demonstrated. Bio-Oss only in combination with PRGF increases the alkaline phosphatase (ALP) activity in EtOH pretreated cells.
CONCLUSIONS
These results indicate that the simultaneous application of PRGF and Bio-Oss inhibits EtOH induced bone healing impairment. Furthermore, in the cells, PRGF induced a protective mechanism which might promote bone regeneration.
背景
长期饮酒是已知的影响骨愈合的限制因素。在口腔颌面外科手术中，一种有望改善使用Bio-Oss进行骨增量技术的策略可能是支持性应用富含血小板的生物材料，即血小板释放生长因子（PRGF）。为了探讨这一问题，我们进行了一项体外研究，调查PRGF和Bio-Oss对乙醇（EtOH）处理的成骨细胞的保护作用。
方法
使用经乙醇预处理和未经预处理的SAOS-2骨肉瘤细胞系。评估在应用0%、5%和10%的PRGF和Bio-Oss后细胞的活力、增殖和碱性磷酸酶活性（ALP）。
结果
在乙醇处理的成骨细胞中应用PRGF和Bio-Oss显示出显著的有益影响，提高了细胞培养中成骨细胞的活力。还证明了Bio-Oss与5%PRGF对成骨细胞增殖的协同作用。仅Bio-Oss与PRGF联合可增加乙醇预处理细胞中的碱性磷酸酶（ALP）活性。
结论
这些结果表明，同时应用PRGF和Bio-Oss可抑制乙醇诱导的骨愈合损伤。此外，在细胞中，PRGF诱导了一种可能促进骨再生的保护机制。
Zotero 插件