• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

膜接触位点处的固醇转运蛋白调节TORC1和TORC2信号传导。

Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling.

作者信息

Murley Andrew, Yamada Justin, Niles Bradley J, Toulmay Alexandre, Prinz William A, Powers Ted, Nunnari Jodi

机构信息

Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA.

National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD.

出版信息

J Cell Biol. 2017 Sep 4;216(9):2679-2689. doi: 10.1083/jcb.201610032. Epub 2017 Aug 3.

DOI:10.1083/jcb.201610032
PMID:28774891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5584152/
Abstract

Membrane contact sites (MCSs) function to facilitate the formation of membrane domains composed of specialized lipids, proteins, and nucleic acids. In cells, membrane domains regulate membrane dynamics and biochemical and signaling pathways. We and others identified a highly conserved family of sterol transport proteins (Ltc/Lam) localized at diverse MCSs. In this study, we describe data indicating that the yeast family members Ltc1 and Ltc3/4 function at the vacuole and plasma membrane, respectively, to create membrane domains that partition upstream regulators of the TORC1 and TORC2 signaling pathways to coordinate cellular stress responses with sterol homeostasis.

摘要

膜接触位点（MCSs）的功能是促进由特殊脂质、蛋白质和核酸组成的膜结构域的形成。在细胞中，膜结构域调节膜动力学以及生化和信号通路。我们和其他研究人员鉴定出了一个高度保守的固醇转运蛋白家族（Ltc/Lam），它们定位于不同的膜接触位点。在本研究中，我们描述了相关数据，表明酵母家族成员Ltc1和Ltc3/4分别在液泡和质膜发挥作用，以创建膜结构域，这些膜结构域将TORC1和TORC2信号通路的上游调节因子分隔开来，从而使细胞应激反应与固醇稳态相协调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/6223df887e47/JCB_201610032_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/a3f040c4edbe/JCB_201610032_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/f56c9352c87b/JCB_201610032_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/adfb15e4ad5d/JCB_201610032_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/4a37678a7889/JCB_201610032_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/6223df887e47/JCB_201610032_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/a3f040c4edbe/JCB_201610032_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/f56c9352c87b/JCB_201610032_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/adfb15e4ad5d/JCB_201610032_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/4a37678a7889/JCB_201610032_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00d2/5584152/6223df887e47/JCB_201610032_Fig5.jpg

相似文献

1
Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling.膜接触位点处的固醇转运蛋白调节TORC1和TORC2信号传导。
J Cell Biol. 2017 Sep 4;216(9):2679-2689. doi: 10.1083/jcb.201610032. Epub 2017 Aug 3.
2
Plasma membrane proteins Slm1 and Slm2 mediate activation of the AGC kinase Ypk1 by TORC2 and sphingolipids in S. cerevisiae.质膜蛋白 Slm1 和 Slm2 介导 TORC2 和鞘脂在酿酒酵母中激活 AGC 激酶 Ypk1。
Cell Cycle. 2012 Oct 15;11(20):3745-9. doi: 10.4161/cc.21752. Epub 2012 Aug 16.
3
Tiered assembly of the yeast Far3-7-8-9-10-11 complex at the endoplasmic reticulum.内质网上酵母 Far3-7-8-9-10-11 复合物的分层组装。
J Biol Chem. 2013 Jun 7;288(23):16986-16997. doi: 10.1074/jbc.M113.451674. Epub 2013 Apr 26.
4
TOR complex 2-regulated protein kinase Ypk1 controls sterol distribution by inhibiting StARkin domain-containing proteins located at plasma membrane-endoplasmic reticulum contact sites.TOR 复合物 2 调节的蛋白激酶 Ypk1 通过抑制位于质膜-内质网接触位点的具有 StAR 激酶结构域的蛋白来控制固醇分布。
Mol Biol Cell. 2018 Aug 15;29(17):2128-2136. doi: 10.1091/mbc.E18-04-0229. Epub 2018 Jun 21.
5
Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2.雷帕霉素不敏感的靶蛋白复合物 2 的分子基础。
Mol Cell. 2015 Jun 18;58(6):977-88. doi: 10.1016/j.molcel.2015.04.031. Epub 2015 May 28.
6
TORC2 and eisosomes are spatially interdependent, requiring optimal level of phosphatidylinositol 4, 5-bisphosphate for their integrity.TORC2与胞膜窖在空间上相互依存，其完整性需要磷脂酰肌醇4,5-二磷酸达到最佳水平。
J Biosci. 2015 Jun;40(2):299-311. doi: 10.1007/s12038-015-9526-4.
7
Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts.Ltc1是一种定位于内质网的固醇转运蛋白，也是内质网-线粒体和内质网-液泡接触位点的组成部分。
J Cell Biol. 2015 May 25;209(4):539-48. doi: 10.1083/jcb.201502033. Epub 2015 May 18.
8
Ubiquitin regulates TORC1 in yeast Saccharomyces cerevisiae.泛素在酿酒酵母中调节雷帕霉素靶蛋白复合体1（TORC1）。
Mol Microbiol. 2016 Apr;100(2):303-14. doi: 10.1111/mmi.13319. Epub 2016 Mar 10.
9
TOR complex 2-Ypk1 signaling is an essential positive regulator of the general amino acid control response and autophagy.TOR 复合物 2-Ypk1 信号是一般氨基酸控制反应和自噬的必需正向调节因子。
Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10586-91. doi: 10.1073/pnas.1406305111. Epub 2014 Jul 7.
10
Gad8 Protein Is Found in the Nucleus Where It Interacts with the MluI Cell Cycle Box-binding Factor (MBF) Transcriptional Complex to Regulate the Response to DNA Replication Stress.Gad8蛋白存在于细胞核中，在那里它与MluI细胞周期盒结合因子（MBF）转录复合物相互作用，以调节对DNA复制应激的反应。
J Biol Chem. 2016 Apr 22;291(17):9371-81. doi: 10.1074/jbc.M115.705251. Epub 2016 Feb 24.

引用本文的文献

1
Valproate independently activates Snf1, inhibits TORC1, and induces repression of INO1 transcription by increasing nuclear localization of Opi1.丙戊酸盐可独立激活Snf1，抑制TORC1，并通过增加Opi1的核定位来诱导INO1转录的抑制。
Sci Rep. 2025 Jul 9;15(1):24601. doi: 10.1038/s41598-025-07540-2.
2
The molecular logic of Gtr1/2- and Pib2-dependent TORC1 regulation in budding yeast.芽殖酵母中Gtr1/2和Pib2依赖性TORC1调控的分子逻辑
Elife. 2025 Jul 7;13:RP94628. doi: 10.7554/eLife.94628.
3
Profiling the LAM Family of Contact Site Tethers Provides Insights into Their Regulation and Function.

本文引用的文献

1
Lysosomal cholesterol activates mTORC1 via an SLC38A9-Niemann-Pick C1 signaling complex.溶酶体胆固醇通过SLC38A9-尼曼-皮克病C1型信号复合物激活mTORC1。
Science. 2017 Mar 24;355(6331):1306-1311. doi: 10.1126/science.aag1417.
2
Size-dependent protein segregation at membrane interfaces.膜界面处的尺寸依赖性蛋白质分离
Nat Phys. 2016 Jul;12(7):704-711. doi: 10.1038/nphys3678. Epub 2016 Mar 7.
3
Yeast cell wall integrity sensors form specific plasma membrane microdomains important for signalling.酵母细胞壁完整性传感器形成对信号传导很重要的特定质膜微区。
分析接触位点系链的LAM家族可深入了解其调控和功能。
Contact (Thousand Oaks). 2025 Apr 17;8:25152564251321770. doi: 10.1177/25152564251321770. eCollection 2025 Jan-Dec.
4
Activation of the cell wall integrity pathway negatively regulates TORC2-Ypk1/2 signaling through blocking eisosome disassembly in Saccharomyces cerevisiae.细胞壁完整性途径的激活通过阻止酿酒酵母中 eisosome 的解体，负调控 TORC2-Ypk1/2 信号通路。
Commun Biol. 2024 Jun 11;7(1):722. doi: 10.1038/s42003-024-06411-2.
5
Membrane contact sites regulate vacuolar fission via sphingolipid metabolism.膜接触位点通过神经酰胺代谢调节液泡分裂。
Elife. 2024 Mar 27;12:RP89938. doi: 10.7554/eLife.89938.
6
The Orf9b protein of SARS-CoV-2 modulates mitochondrial protein biogenesis.SARS-CoV-2 的 Orf9b 蛋白调节线粒体蛋白的生物发生。
J Cell Biol. 2023 Oct 2;222(10). doi: 10.1083/jcb.202303002. Epub 2023 Sep 8.
7
The phosphatase Glc7 controls the eisosomal response to starvation via post-translational modification of Pil1.磷酸酶 Glc7 通过 Pil1 的翻译后修饰控制饥饿诱导的内体反应。
J Cell Sci. 2023 Jul 15;136(14). doi: 10.1242/jcs.260505. Epub 2023 Jul 24.
8
Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases).酵母液泡膜脂类组从对数（一相）到静止期（二相）的重塑。
Biophys J. 2023 Mar 21;122(6):1043-1057. doi: 10.1016/j.bpj.2023.01.009. Epub 2023 Jan 12.
9
Fundamental roles for inter-organelle communication in aging.细胞器间通讯在衰老中的基本作用。
Biochem Soc Trans. 2022 Oct 31;50(5):1389-1402. doi: 10.1042/BST20220519.
10
TOR complex 2 is a master regulator of plasma membrane homeostasis.TOR 复合物 2 是质膜稳态的主要调节因子。
Biochem J. 2022 Sep 30;479(18):1917-1940. doi: 10.1042/BCJ20220388.
Cell Microbiol. 2016 Sep;18(9):1251-67. doi: 10.1111/cmi.12635. Epub 2016 Jul 22.
4
Phase separation of signaling molecules promotes T cell receptor signal transduction.信号分子的相分离促进T细胞受体信号转导。
Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. Epub 2016 Apr 7.
5
Dynein Clusters into Lipid Microdomains on Phagosomes to Drive Rapid Transport toward Lysosomes.动力蛋白在吞噬体上聚集形成脂质微区，以驱动向溶酶体的快速运输。
Cell. 2016 Feb 11;164(4):722-34. doi: 10.1016/j.cell.2015.12.054. Epub 2016 Feb 4.
6
Down-regulation of TORC2-Ypk1 signaling promotes MAPK-independent survival under hyperosmotic stress.在高渗应激下，TORC2 - Ypk1信号通路的下调促进不依赖丝裂原活化蛋白激酶的细胞存活。
Elife. 2015 Aug 14;4:e09336. doi: 10.7554/eLife.09336.
7
Lam6 Regulates the Extent of Contacts between Organelles.Lam6调节细胞器之间的接触程度。
Cell Rep. 2015 Jul 7;12(1):7-14. doi: 10.1016/j.celrep.2015.06.022. Epub 2015 Jun 25.
8
Conserved SMP domains of the ERMES complex bind phospholipids and mediate tether assembly.ERMES复合物保守的SMP结构域可结合磷脂并介导系链组装。
Proc Natl Acad Sci U S A. 2015 Jun 23;112(25):E3179-88. doi: 10.1073/pnas.1422363112. Epub 2015 Jun 8.
9
A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport.膜接触位点处的一类新的StART结构域蛋白在内质网-质膜固醇转运中发挥作用。
Elife. 2015 May 22;4:e07253. doi: 10.7554/eLife.07253.
10
The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis.I-BAR 蛋白 Ivy1 是 Rab7 GTP 酶 Ypt7 的效应因子，参与液泡膜的稳态。
J Cell Sci. 2015 Jul 1;128(13):2278-92. doi: 10.1242/jcs.164905. Epub 2015 May 21.