Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, Honolulu, HI 96822, United States.
Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, Honolulu, HI 96822, United States.
Placenta. 2017 Nov;59 Suppl 1(Suppl 1):S32-S36. doi: 10.1016/j.placenta.2017.07.016. Epub 2017 Jul 29.
The placenta is an organ vital to fetal development as well as the maintenance of a healthy pregnancy and plays a crucial role in developmental programming of the fetus. The mechanisms that link intrauterine milieu, fetal health and disease development later in life are poorly understood. Placenta-specific gene modulation, both by generating transgenic animals as well as by developing methods for in vivo genetic modifications is a growing area of interest as this approach provides the opportunity to investigate the role of particular genes or gene networks in regulating placental function and fetal growth. Furthermore, in vivo placental gene transfer may be adapted to treat humans in the future and could be used as an early intervention strategy for a wide range of pregnancy complications. This review is an overview of transposase-based methods available for both transgenic animal generation and in vivo placental gene modifications with an emphasis on piggyBac-based systems.
胎盘是胎儿发育以及维持健康妊娠所必需的器官，在胎儿发育编程中起着至关重要的作用。将宫内环境、胎儿健康和日后疾病发展联系起来的机制还知之甚少。通过生成转基因动物以及开发体内遗传修饰方法来调节胎盘特异性基因，这是一个日益受到关注的领域，因为这种方法提供了研究特定基因或基因网络在调节胎盘功能和胎儿生长中的作用的机会。此外，体内胎盘基因转移将来可能适用于人类治疗，并可作为治疗各种妊娠并发症的早期干预策略。本文综述了可用于转基因动物生成和体内胎盘基因修饰的转座酶方法，重点介绍了基于 piggyBac 的系统。
