Department of Emergency, Yishui Central Hospital of Linyi, Linyi 276400, Shandong Province, P.R. China.
Department of Emergency, Yishui Central Hospital of Linyi, Linyi 276400, Shandong Province, P.R. China
Biosci Rep. 2017 Aug 23;37(4). doi: 10.1042/BSR20160612. Print 2017 Aug 31.
We aim to explore the associations between matrix metalloproteinase (MMP) gene polymorphism with ulinastatin (UTI) efficacy in treating severe acute pancreatitis (SAP). A total of 276 SAP patients were assigned into the control (=135) and observation (=141) groups. PCR-restriction fragment length polymorphism (PCR-RFLP) was used for genotype and allele frequency distribution. Relevance of genotypes with UTI efficacy was analyzed. The observation group showed lowered duration in symptoms (abdominal distension, abdominal pain, tenderness, and rebound tenderness) than the control group. Laboratory analysis (serum calcium, white blood cells, serum amylase, urine amylase, APACHE-II, and Balthazar CTIS scores) were decreased, while serum albumin levels increased after 7th day of therapy. The total effective rate of UTI for patients with C-1306T C/C genotype was higher than those with C/T and T/T genotypes after the 7th day of therapy, which was lower in patients with C-1562T C/C and C/T genotypes than those with T/T genotype. The duration for symptoms in patients with C-1562T T/T genotype was shorter than those with C/C and C/T genotypes, which was less in patients with C-1306T C/C genotype than those with C/T and T/T genotypes. The improvement values of APACHE-II and Balthazar CTIS scores for patients with C-1306T C/C genotype were higher than those with C/T and T/T genotypes, which for patients with C-1562T C/C and C/T genotypes were lower than those with T/T genotype. These results demonstrated that gene polymorphism was associated with UTI efficacy for SAP.
我们旨在探讨基质金属蛋白酶（MMP）基因多态性与乌司他丁（UTI）治疗重症急性胰腺炎（SAP）疗效的关系。将 276 例 SAP 患者分为对照组（n=135）和观察组（n=141）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测基因型和等位基因频率分布。分析基因型与 UTI 疗效的相关性。观察组的症状（腹胀、腹痛、压痛和反跳痛）持续时间短于对照组。实验室分析（血清钙、白细胞、血清淀粉酶、尿淀粉酶、APACHE-II 和 Balthazar CTIS 评分）下降，治疗第 7 天血清白蛋白水平升高。第 7 天治疗后，C-1306T C/C 基因型患者 UTI 的总有效率高于 C/T 和 T/T 基因型，C-1562T C/C 和 C/T 基因型患者的总有效率低于 T/T 基因型。C-1562T T/T 基因型患者的症状持续时间短于 C/C 和 C/T 基因型，C-1306T C/C 基因型患者的症状持续时间短于 C/T 和 T/T 基因型。C-1306T C/C 基因型患者的 APACHE-II 和 Balthazar CTIS 评分改善值高于 C/T 和 T/T 基因型，C-1562T C/C 和 C/T 基因型患者的改善值低于 T/T 基因型。这些结果表明基因多态性与 SAP 患者 UTI 疗效相关。
