Oliveira Thaís Larré, Rizzi Caroline, Dellagostin Odir Antônio
Programa de Pós-Graduação em Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Unidade de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, Pelotas, RS, CEP 96010-900, Brazil.
Appl Microbiol Biotechnol. 2017 Sep;101(18):6865-6877. doi: 10.1007/s00253-017-8439-6. Epub 2017 Aug 4.
Recombinant Mycobacterium bovis BCG vaccines (rBCG) were first developed in the 1990s as a means of expressing antigens from multiple pathogens. This review examines the key structural factors of recombinant M. bovis that influence the expression of the heterologous antigens and the generation of genetic and functional stability in rBCG, which are crucial for inducing strong and lasting immune responses. The fundamental aim of this paper is to provide an overview of factors that affect the expression of recombinant proteins in BCG and the generation of the immune response against the target antigens, including mycobacterial promoters, location of foreign antigens, and stability of the vectors. The reporter systems that have been employed for evaluation of these molecular features in BCG are also reviewed here.
重组牛分枝杆菌卡介苗（rBCG）于20世纪90年代首次开发，作为表达多种病原体抗原的一种手段。本综述探讨了重组牛分枝杆菌的关键结构因素，这些因素影响异源抗原的表达以及rBCG中遗传和功能稳定性的产生，而这对于诱导强烈而持久的免疫反应至关重要。本文的基本目的是概述影响卡介苗中重组蛋白表达以及针对靶抗原产生免疫反应的因素，包括分枝杆菌启动子、外源抗原的位置和载体的稳定性。本文还综述了用于评估卡介苗中这些分子特征的报告系统。
