Low M J, Stork P J, Hammer R E, Brinster R L, Warhol M J, Mandel G, Goodman R H
J Biol Chem. 1986 Dec 5;261(34):16260-3.
The pituitaries of transgenic mice that express a metallothionein-somatostatin fusion gene contain high concentrations of somatostatin-14 exclusively in the gonadotrophic cells. The purpose of this study was to determine whether somatostatin expressed from the foreign fusion gene enters the normal secretory pathway within these cells. Immuno-gold labeling of serial thin sections localized somatostatin to the secretory granules of gonadotropin-producing cells. The gonadotroph-specific hypophysiotropic factor, luteinizing hormone-releasing hormone caused a dose-dependent secretion of somatostatin when applied to primary pituitary cultures from these mice. Growth hormone-releasing hormone, thyrotropin-releasing hormone, corticotropin releasing factor, and dopamine did not affect somatostatin secretion. These experiments demonstrate that a neurosecretory peptide encoded by a foreign gene can enter the regulated secretory pathway of pituitary cells from transgenic mice.
表达金属硫蛋白-生长抑素融合基因的转基因小鼠的垂体中，生长抑素-14仅在促性腺激素细胞中高浓度存在。本研究的目的是确定从外源融合基因表达的生长抑素是否进入这些细胞内的正常分泌途径。对连续薄切片进行免疫金标记，将生长抑素定位到促性腺激素生成细胞的分泌颗粒中。当将促性腺激素特异性的垂体促激素释放因子促黄体生成素释放激素应用于这些小鼠的原代垂体培养物时，会引起生长抑素的剂量依赖性分泌。生长激素释放激素、促甲状腺激素释放激素、促肾上腺皮质激素释放因子和多巴胺不影响生长抑素的分泌。这些实验表明，外源基因编码的神经分泌肽可进入转基因小鼠垂体细胞的调节性分泌途径。
