Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.
Bioresour Technol. 2017 Nov;244(Pt 1):243-251. doi: 10.1016/j.biortech.2017.07.096. Epub 2017 Jul 19.
An alkali-tolerant Chlorella sp. AT1 mutant strain was screened by NTG mutagenesis. The strain grew well in pH 6-11 media, and the optimal pH for growth was 10. The CO utilization efficiencies of Chlorella sp. AT1 cultured with intermittent 10% CO aeration for 10, 20 and 30min at 3-h intervals were approximately 80, 42 and 30%, respectively. In alkaline medium (pH=11) with intermittent 10% CO aeration for 30min at 3-, 6- and 12-h intervals, the medium pH gradually changed to 10, and the biomass productivities of Chlorella sp. AT1 were 0.987, 0.848 and 0.710gLd, respectively. When Chlorella sp. AT1 was aerated with 10% CO intermittently for 30min at 3-h intervals in semi-continuous cultivation for 21days, the biomass concentration and biomass productivity were 4.35gL and 0.726gLd, respectively. Our results show that CO utilization efficiency can be markedly increased by intermittent CO aeration and alkaline media as a CO-capturing strategy for alkali-tolerant microalga cultivation.
通过 NTG 诱变筛选出一株耐碱小球藻 AT1 突变株。该菌株在 pH6-11 的培养基中生长良好，最适生长 pH 为 10。当以 3 小时间隔间歇通入 10%CO2 通气 10、20 和 30min 时，间歇通入 10%CO2 通气培养的小球藻 AT1 的 CO 利用效率分别约为 80%、42%和 30%。在 pH=11 的碱性培养基中，以 3、6 和 12 小时间隔间歇通入 10%CO2 通气 30min，培养基 pH 逐渐变为 10，小球藻 AT1 的生物量生产力分别为 0.987、0.848 和 0.710gLd。当小球藻 AT1 在半连续培养 21 天的间歇 3 小时间隔通入 10%CO2 通气 30min 时，生物量浓度和生物量生产力分别为 4.35gL 和 0.726gLd。我们的结果表明，通过间歇 CO2 通气和碱性介质作为耐碱微藻培养的 CO 捕获策略，可以显著提高 CO 利用效率。
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