The indigenous microalga Chlorella sorokiniana NBU-3 grown under air, 5 %, 15 %, and 25 % CO supply was evaluated to determine its potential for flue gas bio-capture, nutrient removal capacity and biomass yield using non-sterilized wastewater as growth medium. The results indicated that C. sorokiniana NBU-3 exhibited high nutrient removal efficiency (>95 % for NH-N, TN and TP) with either air or CO aeration. 5 %-15 % CO supplies promote biomass yield, nutrient utilization and CO biofixation of C. sorokiniana NBU-3. In particular, 15 % CO promotes C. sorokiniana NBU-3 growth in non-sterilized MW, but inhibits its growth in BG11 medium, indicating the importance of non-sterilized MW and high CO aeration concurrence for C. sorokiniana NBU-3 economically practical cultivation. Moreover, the highest values of lipid (27.84 ± 2.12 %) and protein (32.65 ± 4.11 %) contents were obtained in MW with 15 % CO aeration. Conceivably, microalgal-bacterial symbiosis may help C. sorokiniana NBU-3 tolerate high concentration of CO and promote microalga growth. The succession of the community diversity toward the specific functional bacterial species such as Methylobacillus and Methylophilus (Proteobacteria) which were predicted to possess the function of methylotroph, methanol oxidation and ureolysis would help facilitate the microalgal-bacterial symbiosis and promote the microalgae biomass accumulation with high dosage of CO aeration. Overall, these findings clearly highlight the potential of this indigenous microalga C. sorokiniana NBU-3 for industrial-emission level CO mitigation and commercial microalga biomass production in MW.