葡萄糖转运蛋白8（GLUT8）在成年公猪睾丸精子发生过程中的表达

Expression of glucose transporter 8 (GLUT8) in spermatogenesis of adult boar testes.

作者信息

Xi Huaming, Zhang Zhen, Ji Rui, Shen Hui, Fan Xiaorui, Li Qinghong, Bai Xiyun, Cheng Zhixue, He Junping

机构信息

Institute of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, PR China.

出版信息

Acta Histochem. 2017 Jul;119(6):632-637. doi: 10.1016/j.acthis.2017.07.004. Epub 2017 Aug 4.

DOI:10.1016/j.acthis.2017.07.004

PMID:28780960

Abstract

The present study was designed to investigate the glucose transporter 8 (GLUT8) expression and localization in adult boar testis. Localization and expression of GLUT8 were conducted with Western Blotting, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods GLUT8 protein and mRNA were expressed in the boar testes. The results of Western Blotting analysis showed specificity of the antibody for protein of boar testes. The immunohistochemistry results showed that GLUT8 protein mainly localized in spermatocytes, round spermatids and elongated spermatids of the seminiferous tubules in the adult boar testes. And the GLUT8 expression persists during eight stages of boar spermatogenesis. GLUT8 may mainly provide glucose for the later stage of germ cell differentiation in adluminal compartment in adult boar testes. These results suggested that GLUT8 is important for the spermatogenesis in the adult boar testes.

摘要

本研究旨在调查成年公猪睾丸中葡萄糖转运蛋白8（GLUT8）的表达及定位情况。采用蛋白质免疫印迹法、免疫组织化学法和逆转录聚合酶链反应（RT-PCR）法对GLUT8进行定位和表达分析。结果显示，GLUT8蛋白质和mRNA在公猪睾丸中均有表达。蛋白质免疫印迹分析结果表明，该抗体对猪睾丸蛋白质具有特异性。免疫组织化学结果显示，GLUT8蛋白主要定位于成年公猪睾丸生精小管的精母细胞、圆形精子细胞和长形精子细胞中。并且在公猪精子发生的八个阶段中，GLUT8的表达持续存在。GLUT8可能主要为成年公猪睾丸管腔内部生殖细胞分化的后期阶段提供葡萄糖。这些结果表明，GLUT8对成年公猪睾丸的精子发生具有重要作用。