Ren Yuxin, Zhao Song, Chen He, Fu Ying-Mei, Zhao Bai
Department of Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Oncol Lett. 2017 Aug;14(2):2453-2457. doi: 10.3892/ol.2017.6450. Epub 2017 Jun 21.
Inhibitor of growth family member 4 (ING4) is a candidate tumor suppressor that serves important roles in tumor growth and angiogenesis. In the present study ING4 expression was assessed in clear cell renal carcinoma (CCRC) tissues and its association with the progression of CCRC was determined. The expression of ING4 in 125 patients with CCRC was analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot analysis and immunohistochemical methods. A total of 40 adjacent normal renal tissues were used as control samples. The results identified that ING4 expression was positive in 100% of normal renal tissues, but in only 82.3% CCRC samples. RT-qPCR and western blotting results demonstrated that expression levels of ING4 mRNA and protein were significantly decreased in CCRC compared with in normal tissues (P<0.0001). ING4 expression was not associated with sex, age or tumor volume (P>0.05), but was associated with the nuclear grade of renal cancer (P<0.0001), the clinical stage of CCRC (P<0.0001) and lymphatic metastasis (P<0.0001). The results of the present study indicated that dysregulation of ING4 expression may be involved in the initiation and progression of CCRC.
生长抑制因子家族成员4（ING4）是一种候选肿瘤抑制因子，在肿瘤生长和血管生成中发挥重要作用。在本研究中，对透明细胞肾细胞癌（CCRC）组织中的ING4表达进行了评估，并确定了其与CCRC进展的关联。采用逆转录-定量聚合酶链反应（RT-qPCR）、蛋白质印迹分析和免疫组织化学方法分析了125例CCRC患者中ING4的表达。共40例相邻正常肾组织用作对照样本。结果发现，ING4在100%的正常肾组织中表达为阳性，但在CCRC样本中仅为&82.3%。RT-qPCR和蛋白质印迹结果表明，与正常组织相比，CCRC中ING4 mRNA和蛋白质的表达水平显著降低（P<0.0001）。ING4表达与性别、年龄或肿瘤体积无关（P>0.05），但与肾癌的核分级（P<0.0001）、CCRC的临床分期（P<0.0001）和淋巴转移（P<0.0001）有关。本研究结果表明，ING4表达失调可能参与了CCRC的发生和进展。
