Frenster J H, Papalian M M, Masek M A, Frenster J A
J Natl Cancer Inst. 1979 Aug;63(2):331-5.
Five hundred and two cells, selected at random within the pretreatment-involved lymph nodes of patients with the nodular sclerosis type of Hodgkin's disease, were analyzed by electron microscopy for in vivo subcellular activity and intercellular interaction. Subpopulations of neoplastic cells and of lymphocytes were recognized by ultrastructural criteria. Neoplastic cells displayed definite sequences of cell maturation, whereas lymphocytes displayed definite sequences of cell activation. Active lymphocytes were most often found apposed to the more active of the neoplastic cells, and the nuclei of such apposed lymphocytes displayed a progressive preferential asymmetry of distribution of active DNA templates into that half of the lymphocyte nucleus closest to the neoplastic cell. Such close apposition of active polysomal lymphocytes to neoplastic cells may correlate with increased patient survival in Hodgkin's disease.
在结节硬化型霍奇金病患者预处理涉及的淋巴结内随机选取502个细胞，通过电子显微镜分析其体内亚细胞活性和细胞间相互作用。通过超微结构标准识别肿瘤细胞和淋巴细胞的亚群。肿瘤细胞表现出明确的细胞成熟序列，而淋巴细胞表现出明确的细胞激活序列。活性淋巴细胞最常与活性较高的肿瘤细胞并置，且这种并置淋巴细胞的细胞核显示出活性DNA模板逐渐优先不对称分布到淋巴细胞核中最靠近肿瘤细胞的那一半。活性多核糖体淋巴细胞与肿瘤细胞的这种紧密并置可能与霍奇金病患者生存率的提高相关。
