用于 HS 检测的“开启”型荧光探针及其在生物成像中的应用。
"Turn-on" fluorescent probe for detection of HS and its applications in bioimaging.
机构信息
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:8-12. doi: 10.1016/j.saa.2017.08.002. Epub 2017 Aug 3.
A novel fluorescent probe (named YQ-1) containing disulfide-bond coumarin derivative was developed for HS. In response to HS, YQ-1 showed remarkable fluorescent emission enhancement at 462nm. Besides, YQ-1 exhibited higher selectivity, faster response rate, low cytotoxicity and low detection limit (0.052μM). Further, YQ-1 was used to detect the presence of HS level in living A549 cells, indicating YQ-1 has good membrane permeability and fluorescence properties.
一种新型的荧光探针（命名为 YQ-1），含有二硫键的香豆素衍生物，被开发用于检测 HS。针对 HS，YQ-1 在 462nm 处表现出显著的荧光发射增强。此外，YQ-1 表现出更高的选择性、更快的响应速度、低细胞毒性和低检测限（0.052μM）。进一步，YQ-1 被用于检测活 A549 细胞中 HS 水平的存在，表明 YQ-1 具有良好的膜透过性和荧光性质。
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