Frión-Herrera Yahima, Díaz-García Alexis, Ruiz-Fuentes Jenny, Rodríguez-Sánchez Hermis, Maurício Sforcin José
a Department of Microbiology and Immunology , Biosciences Institute, UNESP , Botucatu , Brazil.
b Laboratories of Biofarmaceuticals and Chemistries Productions (LABIOFAM) , Havana , Cuba.
Nat Prod Res. 2018 Sep;32(17):2085-2091. doi: 10.1080/14786419.2017.1363749. Epub 2017 Aug 8.
Propolis has been used as a traditional remedy for centuries because of its beneficial effects, including anticancer properties. The aim of this study was to compare the cytotoxic mechanism of Cuban red propolis (CP) and Brazilian green propolis (BP) on human laryngeal carcinoma (HEp-2) cells. Cell viability, leakage of lactate dehydrogenase, fluorescence staining, mitochondrial membrane potential (ΔΨm) and the expression of pro/anti-apoptotic genes were assessed. Cell viability and cytotoxic assays suggested a dose-dependent effect of CP and BP extracts with a possible association of intracellular reactive oxygen species production and decreased ΔΨm. Both samples induced apoptosis via activation of TP53, CASP3, BAX, P21 signalling, and downregulation of BCL2 and BCL-XL. CP exerted a higher cytotoxic effect than BP extract. Our findings suggest further investigation of the main components of each propolis sample, what may lead to the development of strategies for the treatment of laryngeal cancer.
几个世纪以来，蜂胶因其有益功效，包括抗癌特性，一直被用作传统药物。本研究的目的是比较古巴红蜂胶（CP）和巴西绿蜂胶（BP）对人喉癌（HEp-2）细胞的细胞毒性机制。评估了细胞活力、乳酸脱氢酶泄漏、荧光染色、线粒体膜电位（ΔΨm）以及促凋亡/抗凋亡基因的表达。细胞活力和细胞毒性测定表明，CP和BP提取物具有剂量依赖性效应，可能与细胞内活性氧的产生和ΔΨm降低有关。两种样品均通过激活TP53、CASP3、BAX、P21信号通路以及下调BCL2和BCL-XL诱导细胞凋亡。CP比BP提取物具有更高的细胞毒性作用。我们的研究结果表明，需要进一步研究每种蜂胶样品的主要成分，这可能会导致开发出治疗喉癌的策略。
