Haq A, Sharma A, Ahmad S, Khan N
Trop Med Parasitol. 1986 Sep;37(3):248-50.
During cellular immune responses sensitized lymphocytes release a number of mediators collectively known as 'lymphokines'. The assaying of macrophage spreading inhibition (MSI) factor released by sensitized lymphocytes was employed as an alternative procedure for in vitro detection of cell-mediated immunity (CMI) in animals immunized with amoeba antigen preparation. The MSI test was compared with macrophage migration inhibition test. A well defined CMI was detectable in animals immunized by amoebic antigen in combination with Freund's complete adjuvant (FCA). Macrophage spreading was found greatly inhibited when peritoneal exudate cells from these animals were cultured in the presence of specific antigen. The optimal time for the development of typical reaction was 30 min. Incubation for a longer time resulted in macrophage clumping.
在细胞免疫反应过程中，致敏淋巴细胞释放多种介质，统称为“淋巴因子”。致敏淋巴细胞释放的巨噬细胞游走抑制（MSI）因子的测定，被用作一种替代方法，用于体外检测用阿米巴抗原制剂免疫的动物的细胞介导免疫（CMI）。将MSI试验与巨噬细胞游走抑制试验进行了比较。在用阿米巴抗原与弗氏完全佐剂（FCA）联合免疫的动物中可检测到明确的CMI。当将这些动物的腹腔渗出细胞在特异性抗原存在下培养时，发现巨噬细胞的游走受到极大抑制。典型反应出现的最佳时间为30分钟。孵育时间延长会导致巨噬细胞聚集。
